pcDNA3.2/V5 hsa-mir-205 | Addgene 26312 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pcDNA3.2/V5 hsa-mir-205

26312

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Chiang H, Schoenfeld L, Ruby J, Auyeung V, Spies N, Baek D, et al. Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Genes Dev. 2010;24:992-1009 pubmed 出版商

产品信息

目录号 :

26312

产品名称 :

pcDNA3.2/V5 hsa-mir-205

文章 :

doi	10.1101/gad.1884710
id	3651
pubmed_id	20413612

细菌的抗性 :

氨苄西林

克隆 :

backbone

pcDNA3.2/V5-DEST

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

生长记录 :

pre-miRNA hairpins and the surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

起源 :

主要研究者 :

alt_names

mir 205

mir-205

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	V5 reverse (5 -ACCGAGGAGAGGGTTAGGGAT-3 )
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIRN205, mir-205
gene	MIR205
id	406988

genbank_ids

mutation

name

hsa-mir-205

shRNA_sequence

size

420

species

9606

Homo sapiens