cDNA

ninaE-stops m3-myc

26855

ninaE-stops m3-myc

氨苄西林

To express the stops cDNA under the control of the ninaE promoter, the cDNA (EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end of the wild-type stops cDNA. The wild-type stops cDNAs was subsequently subcloned between the NotI and XbaI sites of pCNX. The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector

37

High Copy