产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pSPL10p::SPL10-绿色荧光蛋白
目录 :
27406
更多信息或购买 :
文章摘录数: 1
参考文献
Nodine M, Bartel D. MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Genes Dev. 2010;24:2678-92 pubmed 出版商
产品信息
目录号 :
27406
产品名称 :
pSPL10p::SPL10-绿色荧光蛋白
文章 :
doi10.1101/gad.1986710
id3947
pubmed_id21123653
细菌的抗性 :
卡那霉素
克隆 :
backboneKAN-GWR-GFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
used to create transgenic lines
生长记录 :
SPL10p::SPL10-GFP constructs were generated by cloning genomic fragments, including 1.7 kb upstream of and including the SPL10 coding sequence (CDS), into pENTR/D-TOPO (Invitrogen), and then recombining these plasmids with Gateway compatible pBIB-KAN-GWR-GFP plasmids using LR clonase (Invitrogen).
生长温度 :
MACH-1 strain of E. coli (from Invitrogen)
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GFP Reverse
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesF17L21.15, F17L21_15
geneAT1G27370
id839626
genbank_ids
mutation
nameSPL10
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on backbone
tagGFP
抗性标记物 :
21
标签 :
未知
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国