产品简要
文章摘录数: 8
| 参考文献 |
|---|
Fanning A, Ma T, Anderson J. Isolation and functional characterization of the actin binding region in the tight junction protein ZO-1. FASEB J. 2002;16:1835-7 pubmed
|
产品信息
目录号 :
30313
产品名称 :
pEGFP表达载体ZO1
文章 :
| doi | 10.1096/fj.02-0121fje |
| id | 4249 |
| pubmed_id | 12354695 |
细菌的抗性 :
卡那霉素
克隆 :
| backbone | pEGFP-C1 | |
| backbone_mutation | ||
| backbone_origin | Clontech | |
| backbone_size | 4735 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
生长记录 :
GCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAA
TCATCCGACTCCTCGTCGGGT
From the author: The insert is an alternatively spliced domain (which we have confirmed in several ways) that is common to the prevalent ZO-1 species in most epithelial cells. It is adjacent to the conserved ZU5 domain. This isoform is not present in all of the cDNAs we have worked with, but we chose to use this isoform for our GFP ZO-1 studies. This is the cDNA and spliceform that I used in all of our studies. The alt splice is actually 60 bp, and is as follows:
TCATCCGACTCCTCGTCGGGT
From the author: The insert is an alternatively spliced domain (which we have confirmed in several ways) that is common to the prevalent ZO-1 species in most epithelial cells. It is adjacent to the conserved ZU5 domain. This isoform is not present in all of the cDNAs we have worked with, but we chose to use this isoform for our GFP ZO-1 studies. This is the cDNA and spliceform that I used in all of our studies. The alt splice is actually 60 bp, and is as follows:
起源 :
37
主要研究者 :
|
抗性标记物 :
| 963 |
标签 :
High Copy
条款 :
| Neomycin (select with G418) |
更多信息或购买 :
公司信息
Addgene
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Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
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