产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pAD43-daf-16 RNAi
目录 :
34833
更多信息或购买 :
Addgene 产品网页
文章摘录数: 4
参考文献
Matilainen O, Ribeiro A, Verbeeren J, Cetinbaş M, Sood H, Sadreyev R, et al. Loss of muscleblind splicing factor shortens Caenorhabditis elegans lifespan by reducing the activity of p38 MAPK/PMK-1 and transcription factors ATF-7 and Nrf/SKN-1. Genetics. 2021;219: pubmed 出版商
Moll L, Roitenberg N, Bejerano Sagie M, Boocholez H, Carvalhal Marques F, Volovik Y, et al. The insulin/IGF signaling cascade modulates SUMOylation to regulate aging and proteostasis in Caenorhabditis elegans. elife. 2018;7: pubmed 出版商
Kitaoka S, Morielli A, Zhao F. FGT-1-mediated glucose uptake is defective in insulin/IGF-like signaling mutants in Caenorhabditis elegans. FEBS Open Bio. 2016;6:576-85 pubmed 出版商
Dillin A, Crawford D, Kenyon C. Timing requirements for insulin/IGF-1 signaling in C. elegans. Science. 2002;298:830-4 pubmed
产品信息
目录号 :
34833
产品名称 :
pAD43-daf-16 RNAi
文章 :
doi10.1126/science.1074240
id5015
pubmed_id12399591
细菌的抗性 :
氨苄西林
克隆 :
backbonepBluescript
backbone_mutationpBluescript was converted into pAD12, with the introduction of T7 promoter at both sides of MCS.
backbone_originStratagene
backbone_size2961
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
RNAi
生长记录 :
Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3None
cloning_site_5None
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F(-20)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_R13H8.1
genedaf-16
id172981
genbank_ids
mutation
namedaf-16
shRNA_sequence
size1600
species
6239
Caenorhabditis elegans
tags
质粒拷贝数 :
Andrew Dillin and Cynthia Kenyon, UCSF.
抗性标记物 :
1146
标签 :
Low Copy
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国