pflaG | Addgene 45477 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pflaG

45477

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Hammer B, Swanson M. Co-ordination of legionella pneumophila virulence with entry into stationary phase by ppGpp. Mol Microbiol. 1999;33:721-31 pubmed

产品信息

目录号 :

45477

产品名称 :

pflaG

文章 :

doi	10.1046/j.1365-2958.1999.01519.x
id	6709
pubmed_id	10447882

细菌的抗性 :

氨苄西林

克隆 :

backbone

pJB98

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

生长记录 :

pflaG was generated by subcloning the 0.8kb XbaI/PstI fragment encoding GFP and T7 gene 10 rbs from pGFPmut3 into pJB98. The flaA promoter including its -35, -10, and transcriptional start sites were amplified from the chromosomal DNA of L. pneumophila 02 and ligated directly upstream of GFP using BamHI/XbaI. pJB98 was generated from pKB5 (Berger and Isberg, 1993) by deletion of ~1.5kb HpaI/EcoRI fragment containing the ptac promoter and lacIq gene.

起源 :

主要研究者 :

alt_names

MB354

cloning

clone_method	Restriction Enzyme
cloning_site_3	PstI
cloning_site_5	BamHI
promoter	flaA
sequencing_primer_3	GFPfusionREV
sequencing_primer_5	aaggatcctttgcgacttttcaaaaaagc
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

flaA-GFP

shRNA_sequence

size

species