产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pAAV-flex-taCasp3-TEVp
目录 :
45580
更多信息或购买 :
文章摘录数: 26
参考文献
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Zhang T, Perkins M, Chang H, Han W, de Araujo I. An inter-organ neural circuit for appetite suppression. Cell. 2022;185:2478-2494.e28 pubmed 出版商
Tsai N, Wang F, Toma K, Yin C, Takatoh J, Pai E, et al. Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nat Neurosci. 2022;25:659-674 pubmed 出版商
Topilko T, Diaz S, Pacheco C, Verny F, Rousseau C, Kirst C, et al. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron. 2022;110:1385-1399.e8 pubmed 出版商
Siemian J, Arenivar M, Sarsfield S, Borja C, Russell C, Aponte Y. Lateral hypothalamic LEPR neurons drive appetitive but not consummatory behaviors. Cell Rep. 2021;36:109615 pubmed 出版商
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产品信息
目录号 :
45580
产品名称 :
pAAV-flex-taCasp3-TEVp
文章 :
doi10.1016/j.cell.2013.04.017
id6799
pubmed_id23663785
细菌的抗性 :
氨苄西林
克隆 :
backbonepAAV-MCS
backbone_mutation
backbone_originStratagene
backbone_size5347
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
Other
Adeno Associated Viral Vector
生长记录 :
The taCasp3-T2A-TEVp transgene was generated by overlapping PCR of plasmids harboring taCasp3 and TEVp (Gray et al., 2010; Cell 142, 637-646 PMID: 20723762). This transgene was inserted in reverse orientation into the plasmid pAAV-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA such that it replaced hChR2(H134R)-EYFP
生长温度 :
For liquid cultures grow in LB:2XYT (1:1) overnight at 30 C.
起源 :
30
主要研究者 :
alt_names
Casp3
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3AAT CAT GTC CCT GCC GTC GAT C
sequencing_primer_5AAA GCA GCG TAT CCA C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCPP32, CPP32B, SCA-1
geneCASP3
id836
genbank_ids
mutationLinker replaced with a TEV protease cleavage site
nameCaspase 3
shRNA_sequence
size847
species
9606
Homo sapiens
100
Synthetic
tags
alt_names
TEV protease
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3AGA GGG GAT CGT TGT AGA AGT C
sequencing_primer_5TTC TCA AGC CTC AGA CAG TGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameTobacco Etch Virus protease
shRNA_sequence
size705
species
tags
抗性标记物 :
1567
1632
标签 :
Low Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国