产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
PU6::unc-119_sgRNA
目录 :
46169
更多信息或购买 :
文章摘录数: 24
参考文献
Wu Y, Ding C, Weinreb A, Manning L, Swaim G, Yogev S, et al. Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. bioRxiv. 2023;: pubmed 出版商
Mathies L, Blackwell G, Bettinger J. New alleles of the SWI/SNF chromatin remodeling complex gene phf-10. MicroPubl Biol. 2022;2022: pubmed 出版商
Goetsch P, Strome S. DREAM interrupted: severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization. Genetics. 2022;221: pubmed 出版商
Singh R, Smit R, Wang X, Wang C, Racher H, Hansen D. Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line. PLoS Genet. 2021;17:e1009687 pubmed 出版商
Ihara S. N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans. MicroPubl Biol. 2021;2021: pubmed 出版商
Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, et al. MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021;49:4171-4185 pubmed 出版商
Zein Sabatto H, Cole T, Hoang H, Tiwary E, Chang C, Miller M. The type II integral ER membrane protein VAP-B homolog in C. elegans is cleaved to release the N-terminal MSP domain to signal non-cell-autonomously. Dev Biol. 2021;470:10-20 pubmed 出版商
Kuo C, Hsu Y, Wang S, Liou B, Lim S, Chen Y, et al. IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans. Front Immunol. 2020;11:561337 pubmed 出版商
Narimatsu T, Ihara S. New allele of C. elegans gene pign-1, named as xyz11. MicroPubl Biol. 2019;2019: pubmed 出版商
Culp E, Richman C, Sharanya D, Jhaveri N, van den Berg W, Gupta B. Genome editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 system. Biol Methods Protoc. 2020;5:bpaa003 pubmed 出版商
Cabral G, Laos T, Dumont J, Dammermann A. Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance. Dev Cell. 2019;50:355-366.e6 pubmed 出版商
Loucks C, Park K, Walker D, McEwan A, Timbers T, Ardiel E, et al. EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling. elife. 2019;8: pubmed 出版商
Aoki I, Tateyama M, Shimomura T, Ihara K, Kubo Y, Nakano S, et al. SLO potassium channels antagonize premature decision making in C. elegans. Commun Biol. 2018;1:123 pubmed 出版商
Chen X, Shibata A, Hendi A, Kurashina M, Fortes E, Weilinger N, et al. Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans. elife. 2018;7: pubmed 出版商
Uebel C, Anderson D, Mandarino L, Manage K, Aynaszyan S, Phillips C. Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci. PLoS Genet. 2018;14:e1007542 pubmed 出版商
Chen B, Liu P, Hujber E, Li Y, Jorgensen E, Wang Z. AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor. Nat Commun. 2017;8:1380 pubmed 出版商
Fernández Cárdenas L, Villanueva Chimal E, Salinas L, José Núñez C, Tuena de Gomez Puyou M, Navarro R. Caenorhabditis elegans ATPase inhibitor factor 1 (IF1) MAI-2 preserves the mitochondrial membrane potential (??m) and is important to induce germ cell apoptosis. PLoS ONE. 2017;12:e0181984 pubmed 出版商
Cottee P, Cole T, Schultz J, Hoang H, Vibbert J, Han S, et al. The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development. Development. 2017;144:2187-2199 pubmed 出版商
Schultz J, Lee S, Cole T, Hoang H, Vibbert J, Cottee P, et al. The secreted MSP domain of C. elegans VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1. Development. 2017;144:2175-2186 pubmed 出版商
Raman P, Zaghab S, Traver E, Jose A. The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Nucleic Acids Res. 2017;45:8463-8473 pubmed 出版商
Choi Y, Edwards L, DiBello A, Jose A. Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs. Nucleic Acids Res. 2017;45:e87 pubmed 出版商
Schwartz M, Jorgensen E. SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Genetics. 2016;202:1277-88 pubmed 出版商
Katic I, Xu L, Ciosk R. CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015;5:1649-56 pubmed 出版商
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产品信息
目录号 :
46169
产品名称 :
PU6::unc-119_sgRNA
文章 :
doi10.1038/nmeth.2532
id6972
pubmed_id23817069
细菌的抗性 :
氨苄西林
克隆 :
backbonepUC57
backbone_mutation
backbone_origin
backbone_size2641
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
生长记录 :
For more information on Calarco Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/calarco/ gRNA target sequence GAATTTTCTGAAATTAAAGA
起源 :
37
主要研究者 :
alt_names
unc-119
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoRI
promoterC. elegans U6 snRNA pol III promoter
sequencing_primer_3M13 (-48) reverse
sequencing_primer_5M13 (-40) forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_M142.1
geneunc-119
id176519
genbank_ids
mutation
nameunc-119 targeting sgRNA
shRNA_sequence
size840
species
100
Synthetic
tags
抗性标记物 :
1609
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国