psd44-GqQL | Addgene 46826 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

psd44-GqQL

46826

更多信息或购买 :

Addgene 产品网页

文章摘录数: 3

参考文献
Cabezudo S, Sanz Flores M, Caballero A, Tasset I, Rebollo E, Díaz A, et al. Gαq activation modulates autophagy by promoting mTORC1 signaling. Nat Commun. 2021;12:4540 pubmed 出版商
Lopez Mosqueda J, Maddi K, Prgomet S, Kalayil S, Marinovic Terzic I, Terzic J, et al. SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks. elife. 2016;5: pubmed 出版商
Hashimoto S, Mikami S, Sugino H, Yoshikawa A, Hashimoto A, Onodera Y, et al. Lysophosphatidic acid activates Arf6 to promote the mesenchymal malignancy of renal cancer. Nat Commun. 2016;7:10656 pubmed 出版商

产品信息

目录号 :

46826

产品名称 :

psd44-GqQL

文章 :

doi
id	6965
pubmed_id

细菌的抗性 :

氨苄西林

克隆 :

backbone

psd44

backbone_mutation

pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252) under control of the PGK promoter.

backbone_origin

Dr. Richard Iggo, University of St Andrews, Edinburgh, UK

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

生长记录 :

cDNA to make this plasmid was obtained from www.cdna.org (ID GNA0Q000C0). Addgene NGS identified an IS1 element in the plasmid sequence, consistent with other psd44 plasmids.

生长株 :

2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

起源 :

主要研究者 :

alt_names

GNAQ

guanine nucleotide binding protein (G protein), q polypeptide

cloning

clone_method	Restriction Enzyme
cloning_site_3	NheI
cloning_site_5	BamHI
promoter	PGK promoter
sequencing_primer_3
sequencing_primer_5	hPGK-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CMAL, CMC1, G-ALPHA-q, GAQ, SWS
gene	GNAQ
id	2776

genbank_ids

mutation

Q209L (The mutation reduces GTPase activity resulting in a constitutively active phenotype)

name

g protein alpha q

shRNA_sequence

size

1120

species

9606

Homo sapiens