产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
5HRE/绿色荧光蛋白
目录 :
46926
更多信息或购买 :
文章摘录数: 16
参考文献
Samuel T, Rapic S, O Brien C, Edson M, Zhong Y, Dacosta R. Quantitative intravital imaging for real-time monitoring of pancreatic tumor cell hypoxia and stroma in an orthotopic mouse model. Sci Adv. 2023;9:eade8672 pubmed 出版商
Sitte Z, Larson T, McIntosh J, Sinanian M, Lockett M. Selecting the appropriate indirect viability assay for 3D paper-based cultures: a data-driven study. Analyst. 2023;148:2245-2255 pubmed 出版商
Zou J, Wang W, Lu Y, Ayala J, Dong K, Zhou H, et al. Neddylation is required for perinatal cardiac development through stimulation of metabolic maturation. Cell Rep. 2023;42:112018 pubmed 出版商
Zeh N, Bräuer M, Raab N, Handrick R, Otte K. Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control. Sci Rep. 2022;12:2268 pubmed 出版商
Mallet C, Cochard J, Leclercq S, Trapp Fragnet L, Chouteau P, Denesvre C. Hypoxia and HIF-1 Trigger Marek's Disease Virus Reactivation in Lymphoma-Derived Latently Infected T Lymphocytes. J Virol. 2021;:JVI0142721 pubmed 出版商
Janská L, Anandi L, Kirchberger N, Marinkovic Z, Schachtner L, Guzelsoy G, et al. The MEMIC is an ex vivo system to model the complexity of the tumor microenvironment. Dis Model Mech. 2021;14: pubmed 出版商
Zeh N, Schlossbauer P, Raab N, Klingler F, Handrick R, Otte K. Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity. Metab Eng Commun. 2021;13:e00181 pubmed 出版商
Ayuso J, Rehman S, Farooqui M, Virumbrales Muñoz M, Setaluri V, Skala M, et al. Microfluidic Tumor-on-a-Chip Model to Study Tumor Metabolic Vulnerability. Int J Mol Sci. 2020;21: pubmed 出版商
Boyce M, Simke W, Kenney R, Lockett M. Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs). Anal Methods. 2020;12:18-24 pubmed 出版商
Park J, Kim M, Kim W, Kwon K, Ha K, Choi B, et al. Isolinderalactone suppresses human glioblastoma growth and angiogenic activity in 3D microfluidic chip and in vivo mouse models. Cancer Lett. 2020;478:71-81 pubmed 出版商
Tan H, Tan S. The focal adhesion protein kindlin-2 controls mitotic spindle assembly by inhibiting histone deacetylase 6 and maintaining α-tubulin acetylation. J Biol Chem. 2020;295:5928-5943 pubmed 出版商
Duette G, Pereyra Gerber P, Rubione J, Pérez P, Landay A, Crowe S, et al. Induction of HIF-1α by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation. MBio. 2018;9: pubmed 出版商
Kim H, Lin Q, Glazer P, Yun Z. The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells. Breast Cancer Res. 2018;20:16 pubmed 出版商
Xia J, Wu H, Lai B, Zheng L, Zhou D, Chang Z, et al. Gene delivery of hypoxia-inducible VEGF targeting collagen effectively improves cardiac function after myocardial infarction. Sci Rep. 2017;7:13273 pubmed 出版商
Truong A, Lockett M. Oxygen as a chemoattractant: confirming cellular hypoxia in paper-based invasion assays. Analyst. 2016;141:3874-82 pubmed 出版商
Vordermark D, Shibata T, Brown J. Green fluorescent protein is a suitable reporter of tumor hypoxia despite an oxygen requirement for chromophore formation. Neoplasia. 2001;3:527-34 pubmed
产品信息
目录号 :
46926
产品名称 :
5HRE/绿色荧光蛋白
文章 :
doi10.1038/sj/neo/7900192
id6991
pubmed_id11774035
细菌的抗性 :
氨苄西林
克隆 :
backbonepEF/myc/cyto
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长记录 :
The 35-bp human VEGF HRE sequence is: CCACAGTGCATACGTGGGCTCCAACAGGTCCTCTT The d2EGFP fragment was amplified from a Clontech (Palo Alto, CA) vector and encodes a destabilized red- shifted variant of wild-type GFP with an excitation maximum of 488 nm, an emission maximum of 507 nm, and a half-life of approximately 2 hours.
生长株 :
hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system
起源 :
37
主要研究者 :
alt_names
VEGFA
VEGF
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoterEF-1 promoter
sequencing_primer_3BGHrev
sequencing_primer_5EF1a-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesL-VEGF, MVCD1, VEGF, VPF
geneVEGFA
id7422
genbank_ids
mutationfive copies of a 35-bp fragment from the hypoxia-responsive element (HRE) of the human VEGF gene and a human cytomegalovirus minimal promoter
name5X HRE of VEGF
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagd2EGFP
抗性标记物 :
1644
1645
标签 :
未知
条款 :
Neomycin (select with G418)
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国