产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
FLAG标签-血球凝集素.1-
目录 :
52512
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, et al. DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell. 2014;28:697-710 pubmed 出版商
产品信息
目录号 :
52512
产品名称 :
FLAG标签-血球凝集素.1-
文章 :
doi10.1016/j.devcel.2014.01.028
id8120
pubmed_id24613396
细菌的抗性 :
氨苄西林
克隆 :
backbonepcDNA3.1-
backbone_mutationadded FLAG-HA tag in front of MCS, tag coding sequence has: Kozak FLAG - 5x Glycine HA 5x Glycine MCS .
backbone_originInvitrogen
backbone_size5462
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长株 :
expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5Xba I
promoterCMV
sequencing_primer_3BGH-polyA reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F25D7.3
geneblmp-1
id172917
genbank_ids
mutationN75S mutation compared GenBank reference sequence NP_001251370.1
nameBlmp-1
shRNA_sequence
size2460
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tagFLAG-HA
质粒拷贝数 :
insert cDNA was amplified from Open Biosystems Cat# OCE1182-7241376 (F25D7.3 C. elegans ORF Clone Caenorhabditis elegans pDONR201)
抗性标记物 :
2465
标签 :
High Copy
条款 :
Neomycin (select with G418)
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国