产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pBJ FLAG标签-hPar1
目录 :
53226
更多信息或购买 :
文章摘录数: 2
参考文献
Gong H, An S, Sassmann A, Liu M, Mastej V, Mittal M, et al. PAR1 Scaffolds TGF?RII to Downregulate TGF-? Signaling and Activate ESC Differentiation to Endothelial Cells. Stem Cell Reports. 2016;7:1050-1058 pubmed 出版商
Ishii K, Hein L, Kobilka B, Coughlin S. Kinetics of thrombin receptor cleavage on intact cells. Relation to signaling. J Biol Chem. 1993;268:9780-6 pubmed
产品信息
目录号 :
53226
产品名称 :
pBJ FLAG标签-hPar1
文章 :
doi
id1091
pubmed_id7683662
细菌的抗性 :
氨苄西林
克隆 :
backbonepBJ1
backbone_mutation
backbone_originMark Davis lab
backbone_size3300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
depositor comments :
The gene for hThrombin Receptor with Flag epitope at its amino terminus. The gene was cloned into the SalI site of pBluescript and excised and inserted into pBJ.
描述 :
mammalian expression of human Par1 with N-terminal FLAG tag
生长温度 :
37
插入 :
alt_names
coagulation factor II (thrombin) receptor
thrombin receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterSV40
sequencing_primer_3EBV-rev
sequencing_primer_5SV40pro F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCF2R, HTR, PAR-1, PAR1, TR
geneF2R
id2149
genbank_ids
mutationDeletion of aa1-39 (native signal peptide)
namePar1
shRNA_sequence
size3200
species
9606
Homo sapiens
tags
locationN terminal on insert
tagbovine prolactin signal sequence (MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS)
locationN terminal on insert
tagFLAG
主要研究者 :
94
质粒拷贝数 :
未知
条款 :
MTA
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国