产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
lenti MS2-P65-HSF1_Hygro
目录 :
61426
更多信息或购买 :
文章摘录数: 114
参考文献
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Zhang Q, Wang S, Li W, Yau E, Hui H, Singh P, et al. Genome-wide CRISPR/Cas9 transcriptional activation screen identifies a histone acetyltransferase inhibitor complex as a regulator of HIV-1 integration. Nucleic Acids Res. 2022;: pubmed 出版商
Huang M, Yang F, Zhang D, Lin M, Duan H, El Mayta R, et al. Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction. Nat Cardiovasc Res. 2022;1:372-388 pubmed 出版商
Feser C, Lees C, Lammers D, Riddle M, Bingham J, Eckert M, et al. Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production. Int J Mol Sci. 2022;23: pubmed 出版商
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Giehrl Schwab J, Giesert F, Rauser B, Lao C, Hembach S, Lefort S, et al. Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons. EMBO Mol Med. 2022;14:e14797 pubmed 出版商
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Luo M, Bai J, Liu B, Yan P, Zuo F, Sun H, et al. H3K18ac Primes Mesendodermal Differentiation upon Nodal Signaling. Stem Cell Reports. 2019;13:642-656 pubmed 出版商
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Chong Z, Ohnishi S, Yusa K, Wright G. Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 2018;19:205 pubmed 出版商
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Lundh M, Plucińska K, Isidor M, Petersen P, Emanuelli B. Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA. Mol Metab. 2017;6:1313-1320 pubmed 出版商
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产品信息
目录号 :
61426
产品名称 :
lenti MS2-P65-HSF1_Hygro
文章 :
doi10.1038/nature14136
id9503
pubmed_id25494202
细菌的抗性 :
氨苄西林
克隆 :
backboneplenti
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
生长记录 :
IMPORTANT NOTES: SAM libraries ordered prior to 4/3/2017 were shipped with this plasmid included. As of 4/3/2017, a version of this plasmid with improved titer is available: Addgene plasmids #89308 lentiMPH v2 ( http://addgene.org/89308 ) For additional information, protocols and an activator sgRNA design tool, visit the Zhang lab website: http://sam.genome-engineering.org/
生长株 :
lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BsiWI
promoterEF1A
sequencing_primer_3cacatagcgtaaaaggagcaacatag
sequencing_primer_5GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationN55K in MS2
nameMS2-P65-HSF1_2A_Hygro
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
抗性标记物 :
898
标签 :
High Copy
条款 :
Hygromycin
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国