产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
CXCR2-Tango
目录 :
66260
更多信息或购买 :
文章摘录数: 2
参考文献
Wang E, Mao T, Klein J, Dai Y, Huck J, Liu F, et al. Diverse Functional Autoantibodies in Patients with COVID-19. medRxiv. 2020;: pubmed 出版商
Kroeze W, Sassano M, Huang X, Lansu K, McCorvy J, Giguère P, et al. PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Nat Struct Mol Biol. 2015;22:362-9 pubmed 出版商
产品信息
目录号 :
66260
产品名称 :
CXCR2-Tango
文章 :
doi10.1038/nsmb.3014
id10377
pubmed_id25895059
细菌的抗性 :
氨苄西林
克隆 :
backboneempty Tango
backbone_mutation
backbone_origin
backbone_size6632
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长记录 :
Please note: Most of the PRESTO-Tango vectors do not contain the XhoI cut site downstream of the tTA sequence and instead carry an XbaI site. If you need to perform an XhoI digest we recommend sequencing with a forward primer at the C-terminus of tTA to determine which of the two sites is present. Suggested primer: 5-gagctccacttagacggcgagg-3. Original backbone was pcDNA3.1(+). These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
生长株 :
Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoterCMV
sequencing_primer_3Tet-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB, WHIMS2
geneCXCR2
id3579
genbank_ids
mutation
nameCXCR2
shRNA_sequence
size1079
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
抗性标记物 :
1493
标签 :
High Copy
条款 :
Neomycin (select with G418)
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国