pPD95.75 RSF-1 | Addgene 66863 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pPD95.75 RSF-1

66863

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y. Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1. Exp Cell Res. 2013;319:1-11 pubmed 出版商

产品信息

目录号 :

66863

产品名称 :

pPD95.75 RSF-1

文章 :

doi	10.1016/j.yexcr.2012.10.008
id	14446
pubmed_id	23103556

细菌的抗性 :

氨苄西林

克隆 :

backbone

PD95.75

backbone_mutation

backbone_origin

Addgene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

生长记录 :

I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.

生长株 :

Generate transgenic C. elegans expressing GFP-fused RSF-1

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	PstI
promoter
sequencing_primer_3	GFP-R primer
sequencing_primer_5	M13 reverse
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_T24F1.3
gene	rsf-1
id	174728

genbank_ids

mutation

name

RSF-1

shRNA_sequence

size

species

6239

Caenorhabditis elegans