产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pLZ31(pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
目录 :
71720
更多信息或购买 :
文章摘录数: 6
参考文献
Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, et al. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics. 2021;: pubmed 出版商
Kurashina M, Wang J, Lin J, Lee K, Johal A, Mizumoto K. Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans. elife. 2021;10: pubmed 出版商
Wirshing A, Cram E. Spectrin regulates cell contractility through production and maintenance of actin bundles in the Caenorhabditis elegans spermatheca. Mol Biol Cell. 2018;29:2433-2449 pubmed 出版商
Kasimatis K, Moerdyk Schauwecker M, Phillips P. Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans. G3 (Bethesda). 2018;8:2655-2662 pubmed 出版商
Liu P, Chen B, Mailler R, Wang Z. Antidromic-rectifying gap junctions amplify chemical transmission at functionally mixed electrical-chemical synapses. Nat Commun. 2017;8:14818 pubmed 出版商
Zhang L, Ward J, Cheng Z, Dernburg A. The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Development. 2015;142:4374-84 pubmed 出版商
产品信息
目录号 :
71720
产品名称 :
pLZ31(pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
文章 :
doi10.1242/dev.129635
id16207
pubmed_id26552885
细菌的抗性 :
氨苄西林
克隆 :
backbonepCFJ151
backbone_mutation
backbone_origin
backbone_size7373
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
depositor comments :
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
描述 :
Expressing an mRuby-tagged TIR1 in the soma of C. elegans
生长温度 :
37
插入 :
alt_names
TIR1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotereef-1A.1 (eft-3)
sequencing_primer_3
sequencing_primer_5ATGCAAAAGAGAATCGCCTTGTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutationcodon-optimized for expression in C. elegans; Two point mutations D170E and M473L incorporated
nameTRANSPORT INHIBITOR RESPONSE 1
shRNA_sequence
size1884
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmRuby
主要研究者 :
2648
质粒拷贝数 :
High Copy
条款 :
MTA
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国