Aurein1.2_(+36GFP)-Cre | Addgene 71748 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

Aurein1.2_(+36GFP)-Cre

71748

更多信息或购买 :

Addgene 产品网页

文章摘录数: 2

参考文献
Wang L, Geng J, Chen L, Guo X, Wang T, Fang Y, et al. Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery. Drug Deliv. 2022;29:386-398 pubmed 出版商
Li M, Tao Y, Shu Y, LaRochelle J, Steinauer A, Thompson D, et al. Discovery and characterization of a peptide that enhances endosomal escape of delivered proteins in vitro and in vivo. J Am Chem Soc. 2015;137:14084-93 pubmed 出版商

产品信息

目录号 :

71748

产品名称 :

Aurein1.2_(+36GFP)-Cre

文章 :

doi	10.1021/jacs.5b05694
id	16192
pubmed_id	26465072

细菌的抗性 :

氨苄西林

克隆 :

backbone

pET29

backbone_mutation

backbone_origin

Novagen

backbone_size

5181

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

生长株 :

Aurein1.2+36GFP-Cre fusion expression in E. Coli

生长温度 :

For protein expression, transform into BL21 Star (DE3) cells. Induce at 1.0 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.

起源 :

主要研究者 :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter
sequencing_primer_3	T7 Terminator
sequencing_primer_5	T7 promoter
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

(GGS)9_Aurein1.2+36GFP-Cre

shRNA_sequence

size

1941

species

100

Synthetic