产品简要
文章摘录数: 1
产品信息
目录号 :
87429
产品名称 :
AICSDP-23:TJP1-mEGFP
文章 :
doi | 10.1091/mbc.E17-03-0209 |
id | 28192202 |
pubmed_id | 28814507 |
细菌的抗性 :
氨苄西林
克隆 :
backbone | pUC57 | ||||
backbone_mutation | |||||
backbone_origin | |||||
backbone_size | |||||
promoter | |||||
sequencing_primer_3 | |||||
sequencing_primer_5 | |||||
vector_types |
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生长记录 :
This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human TJP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRALERDK. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Please visit https://www.biorxiv.org/content/early/2017/03/31/123042 for bioRxiv preprint.
生长株 :
Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
起源 :
37
主要研究者 :
|
抗性标记物 :
3277 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国