产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
YIp204-PADH1-atTIR1-9myc
目录 :
99532
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
John Peter A, Petrungaro C, Peter M, Kornmann B. METALIC reveals interorganelle lipid flux in live cells by enzymatic mass tagging. Nat Cell Biol. 2022;24:996-1004 pubmed 出版商
产品信息
目录号 :
99532
产品名称 :
YIp204-PADH1-atTIR1-9myc
文章 :
doi
id28189939
pubmed_id
细菌的抗性 :
氨苄西林
克隆 :
backboneYIplac204
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
生长记录 :
Digest this vector with EcoRV for integration into TRP1. Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
生长株 :
S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterADH1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutation
nameTIR1
shRNA_sequence
size2909
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tag9xMyc
质粒拷贝数 :
The TIR1 gene was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
抗性标记物 :
3522
标签 :
High Copy
条款 :
TRP1
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国