产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
PsbA D1 protein of PSII, C-terminal (chicken)
目录 :
AS01 016
规格 :
100微升
价格 :
401美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 8
参考文献
Toubiana D, Sade N, Liu L, Rubio Wilhelmi M, Brotman Y, Luzarowska U, et al. Correlation-based network analysis combined with machine learning techniques highlight the role of the GABA shunt in Brachypodium sylvaticum freezing tolerance. Sci Rep. 2020;10:4489 pubmed 出版商
Sevilla E, Sarasa Buisan C, Gonzalez A, Cases R, Kufryk G, Peleato M, et al. Regulation by FurC in Anabaena Links the Oxidative Stress Response to Photosynthetic Metabolism. Plant Cell Physiol. 2019;60:1778-1789 pubmed 出版商
Krupinska K, Braun S, Nia M, Schäfer A, Hensel G, Bilger W. The nucleoid-associated protein WHIRLY1 is required for the coordinate assembly of plastid and nucleus-encoded proteins during chloroplast development. Planta. 2019;249:1337-1347 pubmed 出版商
Figlioli F, Sorrentino M, Memoli V, Arena C, Maisto G, Giordano S, et al. Overall plant responses to Cd and Pb metal stress in maize: Growth pattern, ultrastructure, and photosynthetic activity. Environ Sci Pollut Res Int. 2019;26:1781-1790 pubmed 出版商
Sicora C, Chiș I, Chiș C, Sicora O. Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light-dark cycle. Photosynth Res. 2019;139:461-473 pubmed 出版商
Kong F, Burlacot A, Liang Y, Légeret B, Alseekh S, Brotman Y, et al. Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018;30:1824-1847 pubmed 出版商
Du Z, Lucker B, Zienkiewicz K, Miller T, Zienkiewicz A, Sears B, et al. Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018;30:447-465 pubmed 出版商
Kim S, Kwon C, Song G, Koh H, An G, Paek N. The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018;11:1 pubmed 出版商
产品信息
产品号 :
AS01 016
产品名称 :
PsbA D1 protein of PSII, C-terminal (chicken)
规格 :
100微升
标价 :
401美元
免疫原 :
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
宿主 :
克隆性 :
多克隆
纯度 :
纯化, 总数IgY(鸡蛋黄免疫球蛋白)在PBS pH值8。 含0.02% 叠氮化钠
格式 :
液体
检测过的应用 :
免疫印迹(免疫印迹)
推荐的稀释浓度 :
1 :4000-1 : 8000, 5微克总数蛋白,(免疫印迹)
预计表观分子量 :
38 28-30 kDa
确认的物种反应活性 :
Alaria esculenta, Amphidinium carterae, Anabaena sp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Cyanothece sp. ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp. , Pinus sylvestris, Spartina alterniflora, Solanum lycopresicum, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis, Zea mays
前体活性 :
Algae (brown and red), Conifers, Cryptomonads, Legumes, Stramenopiles, Euglenoids, Prochlorophytes, Xantophytes
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
相关产品 :
Plant and algla protein extraction bufferSecondary antibodies
选取的参考文献 :
Vitale et al.(2022) Manipulation of light quality is an effective tool to regulate photosynthetic capacity and fruit antioxidant properties of Solanum lycopersicum L. cv. 'Microtom' in a controlled environment. PeerJ. 2022;10:e13677. Published 2022 Jul 1. doi:10.7717/peerj.13677Toubiana et al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489Sicora et al. (2019). Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light-dark cycle. Photosynth Res. 2019 Mar;139(1-3):461-473. doi: 10.1007/s11120-018-0598-5,Sevilla et al. (2019). Regulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism. Plant Cell Physiol. 2019 May 21. pii: pcz094. doi: 10.1093/pcp/pcz094.Figlioli et al. (2019). Overall plant responses to Cd and Pb metal stress in maize: Growth pattern, ultrastructure, and photosynthetic activity. Environ Sci Pollut Res Int. 2019 Jan;26(2):1781-1790. doi: 10.1007/s11356-018-3743-y.
背景 :
The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
储存 :
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies.
UniProt数据库数目 :
P83755, P0C434, Q14FH6, P14660, P14660
TAIR数目 :
ATCG00020
Commodity Code :
38220000
Our Category :
Global Antibodies
研究领域 :
植物生物学, 光合作用
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。