产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
RbcL Rubisco large subunit, form I (chicken)
目录 :
AS01 017
规格 :
50微升
价格 :
394美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
文章摘录数: 2
参考文献
Lv R, Li Z, Li M, Dogra V, Lv S, Liu R, et al. Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019;31:210-230 pubmed 出版商
Gellért A, Pósa T, Fábián A, Szabo L, Bóka K, Forró B, et al. A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018;251:47-55 pubmed 出版商
产品信息
产品号 :
AS01 017
产品名称 :
RbcL Rubisco large subunit, form I (chicken)
规格 :
50微升
标价 :
394美元
免疫原 :
KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1
宿主 :
克隆性 :
多克隆
纯度 :
纯化, 总数IgY(鸡蛋黄免疫球蛋白)在PBS pH值8。 含0.02% 叠氮化钠
格式 :
液体
检测过的应用 :
Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)
推荐的稀释浓度 :
(IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 µg of total cellular protein, (WB)
预计表观分子量 :
52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
确认的物种反应活性 :
Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
前体活性 :
Algae, Dicots, Conifers, Liverworts, Mosses, Prochlorophytes, Welwitschia
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, (AS03 037) combined with Rubisco ready to use standard (AS01 017) is recommended.
相关产品 :
Plant and algal protein extraction bufferSecondary antibodies
选取的参考文献 :
Guljamow et al. (2021) Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806. Microorganisms. 2021 Jun 10;9(6):1265. doi: 10.3390/microorganisms9061265. PMID: 34200971; PMCID: PMC8230624. (IF)Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1-20. (sea-ice diatom)Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gellért et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005.Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.
背景 :
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
储存 :
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria
UniProt数据库数目 :
O03042, Q3ALL1
TAIR数目 :
ATCG00490
Commodity Code :
38220000
Our Category :
Global Antibodies
研究领域 :
植物生物学, 光合作用
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。