产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
RbcL Rubisco large subunit, form I (rabbit)
目录 :
AS03 037
规格 :
50微升
价格 :
302美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
Agrisera 产品网页
文章摘录数: 34
参考文献
Cecchin M, Jeong J, Son W, Kim M, Park S, Zuliani L, et al. LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021;107:1648-1662 pubmed 出版商
Li X, Chai Y, Yang H, Tian Z, Li C, Xu R, et al. Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021;8:31 pubmed 出版商
Khajuria M, Rahul V, Vyas D. Photochemical efficiency is negatively correlated with the Δ9- tetrahydrocannabinol content in Cannabis sativa L. Plant Physiol Biochem. 2020;151:589-600 pubmed 出版商
Wang C, Rong H, Zhang X, Shi W, Hong X, Liu W, et al. Effects and mechanisms of foliar application of silicon and selenium composite sols on diminishing cadmium and lead translocation and affiliated physiological and biochemical responses in hybrid rice (Oryza sativa L.) exposed to cadmium and lead. Chemosphere. 2020;251:126347 pubmed 出版商
Kurmayer R, Entfellner E, Weisse T, Offterdinger M, Rentmeister A, Deng L. Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020;10:2781 pubmed 出版商
Zhang N, Zou H, Lin X, Pan Q, Zhang W, Zhang J, et al. Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020;43:1130-1147 pubmed 出版商
Buck J, Sherman J, Bártulos C, Serif M, Halder M, Henkel J, et al. Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. Nat Commun. 2019;10:4167 pubmed 出版商
Ermakova M, López Calcagno P, Raines C, Furbank R, von Caemmerer S. Overexpression of the Rieske FeS protein of the Cytochrome b6f complex increases C4 photosynthesis in Setaria viridis. Commun Biol. 2019;2:314 pubmed 出版商
Saha S, Hafrén A, Mäkinen K. Dynamics of Protein Accumulation from the 3' End of Viral RNA Are Different from Those in the Rest of the Genome in Potato Virus A Infection. J Virol. 2019;93: pubmed 出版商
Saucedo S, Gonzalez A, Gomez M, Contreras R, Laporte D, Sáez C, et al. Oligo-carrageenan kappa increases glucose, trehalose and TOR-P and subsequently stimulates the expression of genes involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus. BMC Plant Biol. 2019;19:258 pubmed 出版商
Schober A, Rï O Bï Rtulos C, Bischoff A, Lepetit B, Gruber A, Kroth P. Organelle Studies and Proteome Analyses of Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana. Plant Cell Physiol. 2019;60:1811-1828 pubmed 出版商
Zavřel T, Faizi M, Loureiro C, Poschmann G, Stühler K, Sinetova M, et al. Quantitative insights into the cyanobacterial cell economy. elife. 2019;8: pubmed 出版商
Deng X, Zhen S, Liu D, Liu Y, Li M, Liu N, et al. Integrated proteome analyses of wheat glume and awn reveal central drought response proteins under water deficit conditions. J Plant Physiol. 2019;232:270-283 pubmed 出版商
Sousa R, Carvalho F, Lima Melo Y, Alencar V, Daloso D, Margis Pinheiro M, et al. Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress. J Exp Bot. 2019;70:627-639 pubmed 出版商
Kong F, Burlacot A, Liang Y, Légeret B, Alseekh S, Brotman Y, et al. Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018;30:1824-1847 pubmed 出版商
Deng X, Liu Y, Xu X, Liu D, Zhu G, Yan X, et al. Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit. Front Plant Sci. 2018;9:425 pubmed 出版商
Diao J, Song X, Zhang X, Chen L, Zhang W. Genetic Engineering of Crypthecodinium cohnii to Increase Growth and Lipid Accumulation. Front Microbiol. 2018;9:492 pubmed 出版商
Pao S, Tsai P, Peng C, Chen P, Tsai C, Yang E, et al. Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018;131:655-670 pubmed 出版商
Ravi A, Guo S, Rasala B, Tran M, Mayfield S, Nikolov Z. Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. Int J Mol Sci. 2018;19: pubmed 出版商
Wu G, Chalvin C, Hoelscher M, Meyer E, Wu X, Bock R. Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED1. Plant Physiol. 2018;176:2472-2495 pubmed 出版商
Kim J, Brown C, Kim M, Burrows E, Bach S, Lun D, et al. Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2017;114:E8007-E8016 pubmed 出版商
Jespersen D, Yu J, Huang B. Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017;8:1224 pubmed 出版商
Lima Neto M, Cerqueira J, da Cunha J, Ribeiro R, Silveira J. Cyclic electron flow, NPQ and photorespiration are crucial for the establishment of young plants of Ricinus communis and Jatropha curcas exposed to drought. Plant Biol (Stuttg). 2017;19:650-659 pubmed 出版商
Baumgart M, Huber I, Abdollahzadeh I, Gensch T, Frunzke J. Heterologous expression of the Halothiobacillus neapolitanus carboxysomal gene cluster in Corynebacterium glutamicum. J Biotechnol. 2017;258:126-135 pubmed 出版商
Tamburino R, Vitale M, Ruggiero A, Sassi M, Sannino L, Arena S, et al. Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017;17:40 pubmed 出版商
Kolesinski P, Rydzy M, Szczepaniak A. Is RAF1 protein from Synechocystis sp. PCC 6803 really needed in the cyanobacterial Rubisco assembly process?. Photosynth Res. 2017;132:135-148 pubmed 出版商
Ribeiro R, Machado E, Magalhães Filho J, Lobo A, Martins M, Silveira J, et al. Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis. J Plant Physiol. 2017;208:61-69 pubmed 出版商
Kwon K, Chan H, León I, Williams Carrier R, Barkan A, Daniell H. Codon Optimization to Enhance Expression Yields Insights into Chloroplast Translation. Plant Physiol. 2016;172:62-77 pubmed 出版商
Herdean A, Teardo E, Nilsson A, Pfeil B, Johansson O, Ünnep R, et al. A voltage-dependent chloride channel fine-tunes photosynthesis in plants. Nat Commun. 2016;7:11654 pubmed 出版商
Wang X, Zhang W, Miao Y, Gao L. Root-Zone Warming Differently Benefits Mature and Newly Unfolded Leaves of Cucumis sativus L. Seedlings under Sub-Optimal Temperature Stress. PLoS ONE. 2016;11:e0155298 pubmed 出版商
Chen Y, Han Y, Zhang M, Zhou S, Kong X, Wang W. Overexpression of the Wheat Expansin Gene TaEXPA2 Improved Seed Production and Drought Tolerance in Transgenic Tobacco Plants. PLoS ONE. 2016;11:e0153494 pubmed 出版商
Meng F, Luo Q, Wang Q, Zhang X, Qi Z, Xu F, et al. Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Sci Rep. 2016;6:23098 pubmed 出版商
Heinnickel M, Kim R, Wittkopp T, Yang W, Walters K, Herbert S, et al. Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016;113:2774-9 pubmed 出版商
Córdoba J, Molina Cano J, Martínez Carrasco R, Morcuende R, Pérez P. Functional and transcriptional characterization of a barley mutant with impaired photosynthesis. Plant Sci. 2016;244:19-30 pubmed 出版商
产品信息
产品号 :
AS03 037
产品名称 :
RbcL Rubisco large subunit, form I (rabbit)
规格 :
50微升
标价 :
302美元
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
免疫原 :
KLH-conjugated synthetic peptide conserved across all known plant, algal and cyanobacterial RbcL protein sequences (form I L8S8 and form II L2), including, Arabidopsis thaliana O03042, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5
宿主 :
兔
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
检测过的应用 :
Immunofluorescence/confocal Immunolocalization (IL) (IF), Immunogold (IG), Tissue Printing (TP), Western blot (WB)
推荐的稀释浓度 :
Immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request, 1: 800 (TP), 1: 5000 - 10 000 (WB)
预计表观分子量 :
52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
确认的物种反应活性 :
Agostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata (benthic foraminifer), Beta vulgaris, Begonia sp., Bienertia sinuspersici, Brassica napus, Kandelia candel, Cannabis sativa L., Chaetoceros furcellatus, Colobanthus quitensis, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margarita Swingle, Fraxinus mandshurica, Fucus vesiculosus, Gladieria sulphuraria, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis (C.C.Davis) s) G.Hansen & Ø.Moestrup (Wilson isolate), Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybrida cv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis,, Porphyra sp., Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharum sp., Schima superba, Skeletonema costatum (diatom), Skeletonema marinoi (diatom), Solanum lycopersicum, Spinacia oleracea, lichens, Stanleya pinnata, Symbiodinium sp., Synechococcus PCC 7942, Synechococcus elongatus UTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
前体活性 :
Alpha proteobacteria, Algae (brown and red), Dicots, Benincasa hispida, Kalanchoe fedtschenkoi; Beta-proteobacteria, Chlorella vulgaris, Conifers, Cryptomonads, Cyanobacteria (prochlorophytes), Gamma-proeobacteria, Liverworts, Manihot esculenta, Monocots, Mosses, Suaeda glauca, Welwitschia; Nannochloropsis sp., Zosteria marinaFor detection in Rhodospirillaceae use product AS15 2955
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
This antibody was used in:Immunocytochemical staining of diatoms according to Schmid (2003) J Phycol 39: 139-153 and Wordemann et al. (1986) J Cell Biol 102: 1688-1698.Immunofluorescence Dreier et al. (2012). FEMS Microbial Ecol., March 2012.Western blot and tissue printing during a student course Ma et al. (2009).As a loading control Sun et al. (2020).Protocol for Rubisco quantification using this antibody can be found here.
相关产品 :
Plant and algal protein extraction bufferSecondary antibodies
选取的参考文献 :
Chen et al. (2023) Producing fast and active Rubisco in tobacco to enhance photosynthesis. Plant Cell. 2023;35(2):795-807. doi:10.1093/plcell/koac348Garcia et al. (2023) Effects of RuBisCO and CO2 concentration on cyanobacterial growth and carbon isotope fractionation [published online ahead of print, 2023 Jan 5]. Geobiology. 2023;10.1111/gbi.12543. doi:10.1111/gbi.12543Minagawa, Dann. (2023) Extracellular CahB1 from Sodaline magerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal beta-Carbonic Anhydrase in Synechocystis sp. PCC6803. Plants (Basel). 2023;12(2):265. Published 2023 Jan 6. doi:10.3390/plants12020265Vidal-Meireles, et al. (2023)The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas. Plant Cell Environ. 2023;46(2):422-439. doi:10.1111/pce.14488Capo-Bauca et al. (2023). Carbon assimilation in upper subtidal macroalgae is determined by an inverse correlation between Rubisco carboxylation efficiency and CO2 concentrating mechanism effectiveness. New Phytol. 2023;237(6):2027-2038. doi:10.1111/nph.18623
背景 :
This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
重组 :
For reconstitution add 50 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. This antibody is not suitable for use in immunoprecipiation. This product can be sold containing ProClin if requested.
UniProt数据库数目 :
O03042, P05698, P0C510, P00877
TAIR数目 :
ATCG00490
Commodity Code :
38220000
Our Category :
Global Antibodies
研究领域 :
植物生物学, 光合作用
更多信息或购买 :
Agrisera 产品网页
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。