产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
PsbA D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)
目录 :
AS05 084
规格 :
50微升
价格 :
302美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
Agrisera 产品网页
文章摘录数: 21
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 拟南芥; 图 1b
De Michele R, McFarlane H, Parsons H, Meents M, Lao J, González Fernández Niño S, et al. Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016;15:900-13 pubmed 出版商
Cecchin M, Jeong J, Son W, Kim M, Park S, Zuliani L, et al. LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021;107:1648-1662 pubmed 出版商
Pipitone R, Eicke S, Pfister B, Glauser G, Falconet D, Uwizeye C, et al. A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. elife. 2021;10: pubmed 出版商
Khajuria M, Rahul V, Vyas D. Photochemical efficiency is negatively correlated with the Δ9- tetrahydrocannabinol content in Cannabis sativa L. Plant Physiol Biochem. 2020;151:589-600 pubmed 出版商
Kurmayer R, Entfellner E, Weisse T, Offterdinger M, Rentmeister A, Deng L. Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020;10:2781 pubmed 出版商
Theis J, Niemeyer J, Schmollinger S, Ries F, Rütgers M, Gupta T, et al. VIPP2 interacts with VIPP1 and HSP22E/F at chloroplast membranes and modulates a retrograde signal for HSP22E/F gene expression. Plant Cell Environ. 2020;43:1212-1229 pubmed 出版商
Rudenko N, Fedorchuk T, Terentyev V, Dymova O, Naydov I, Golovko T, et al. The role of carbonic anhydrase α-CA4 in the adaptive reactions of photosynthetic apparatus: the study with α-CA4 knockout plants. Protoplasma. 2020;257:489-499 pubmed 出版商
Levitan O, Chen M, Kuang X, Cheong K, Jiang J, Banal M, et al. Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019;116:17316-17322 pubmed 出版商
An F, Chen T, Li Q, Qiao J, Zhang Z, Carvalho L, et al. Protein Cross-Interactions for Efficient Photosynthesis in the Cassava Cultivar SC205 Relative to Its Wild Species. J Agric Food Chem. 2019;67:8746-8755 pubmed 出版商
Dogra V, Li M, Singh S, Li M, Kim C. Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019;10:2834 pubmed 出版商
Schober A, Rï O Bï Rtulos C, Bischoff A, Lepetit B, Gruber A, Kroth P. Organelle Studies and Proteome Analyses of Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana. Plant Cell Physiol. 2019;60:1811-1828 pubmed 出版商
Koh H, Kang N, Jeon S, Shin S, Jeong B, Chang Y. Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels. 2019;12:122 pubmed 出版商
Zavřel T, Faizi M, Loureiro C, Poschmann G, Stühler K, Sinetova M, et al. Quantitative insights into the cyanobacterial cell economy. elife. 2019;8: pubmed 出版商
Lv R, Li Z, Li M, Dogra V, Lv S, Liu R, et al. Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019;31:210-230 pubmed 出版商
Giovanardi M, Pantaleoni L, Ferroni L, Pagliano C, Albanese P, Baldisserotto C, et al. In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta Bioenerg. 2018;: pubmed 出版商
Pao S, Tsai P, Peng C, Chen P, Tsai C, Yang E, et al. Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018;131:655-670 pubmed 出版商
Ananyev G, Gates C, Kaplan A, Dismukes G. Photosystem II-cyclic electron flow powers exceptional photoprotection and record growth in the microalga Chlorella ohadii. Biochim Biophys Acta Bioenerg. 2017;1858:873-883 pubmed 出版商
Míguez F, Fernández Marín B, Becerril J, García Plazaola J. Diversity of winter photoinhibitory responses: a case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017;160:282-296 pubmed 出版商
Sharwood R, Crous K, Whitney S, Ellsworth D, Ghannoum O. Linking photosynthesis and leaf N allocation under future elevated CO2 and climate warming in Eucalyptus globulus. J Exp Bot. 2017;68:1157-1167 pubmed 出版商
Romanowska E, Buczyńska A, Wasilewska W, Krupnik T, Drozak A, Rogowski P, et al. Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta. 2017;245:641-657 pubmed 出版商
An F, Chen T, Stéphanie D, Li K, Li Q, Carvalho L, et al. Domestication Syndrome Is Investigated by Proteomic Analysis between Cultivated Cassava (Manihot esculenta Crantz) and Its Wild Relatives. PLoS ONE. 2016;11:e0152154 pubmed 出版商
产品信息
产品号 :
AS05 084
产品名称 :
PsbA D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)
规格 :
50微升
标价 :
302美元
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
免疫原 :
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
宿主 :
兔
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
检测过的应用 :
Immunofluorescence (IF), ImmunoGold (IG), Western blot (WB)
推荐的稀释浓度 :
1: 500(IF), 1: 200(IgE), 1 : 10 000(免疫印迹)
预计表观分子量 :
38 28-30 kDa
确认的物种反应活性 :
Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp. , Cannabis sativa L., Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis (Kunth) Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex
前体活性 :
Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Cannabis sativa, Galdieria sulphuraria, Lactuca sativa, Lycopersicum esculentum, Medicago sativa, Nannochloropsis sp., Oryza sativa, Ostreococcus sp. Pisum sativum, Sesamum indicum, Thalassiosira pseudonana, Zosteria marina, Vitis vinifera cellular [compartment marker] of thylakoid membrane
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.The peptide is conserved in cyanobacterial D1:1 and D1;2.
相关产品 :
Plant and algal protein extraction buffer Secondary antibodies
选取的参考文献 :
Ivanov et al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Byeon et al. (2022) Canopy height affects the allocation of photosynthetic carbon and nitrogen in two deciduous tree species under elevated CO2. J Plant Physiol. 2022 Jan;268:153584. doi: 10.1016/j.jplph.2021.153584. Epub 2021 Dec 2. PMID: 34890847.Ye et al. (2022) Effect of increased CO2 on iron-light-CO2 co-limitation of growth in a marine diatom, ASLO, Limnol. Oceanogr. 2022, 172-176Pavlovic & Kocab. (2021) Alternative oxidase (AOX) in the carnivorous pitcher plants of the genus Nepenthes: what is it good for? Ann Bot. 2021 Dec 18:mcab151. doi: 10.1093/aob/mcab151. Epub ahead of print. PMID: 34922341.Cano-Ramirez et al. (2021) M. Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells. 2021 Oct 17;10(10):2778. doi: 10.3390/cells10102778. PMID: 34685758; PMCID: PMC8535034.
背景 :
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
重组 :
For reconstitution add 50 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.This product can be sold containing ProClin if requested.
UniProt数据库数目 :
P83755, P0C434, Q14FH6, Q6YXN7, P07753, P14660
TAIR数目 :
ATCG00020
Commodity Code :
38220000
Our Category :
类囊体膜标记
研究领域 :
植物生物学, 光合作用
更多信息或购买 :
Agrisera 产品网页
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。