产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
AtpB Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl)
目录 :
AS05 085-10
规格 :
10微升
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
衣藻, 大鼠, , 拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
产品信息
产品号 :
AS05 085-10
产品名称 :
AtpB Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl)
免疫原 :
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
宿主 :
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
规格 :
10微升
检测过的应用 :
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
相关产品 :
AS05 085 Anti-AtpB beta subunit of ATP synthase (100 µl), rabbit antibodiesAS03 030 Anti-AtpB (developed to exactly the same peptide as rabbit antibody), chicken antibodiesAS03 030S ATP synthase subunit beta protein standard for quantitation and positive controlAS08 304 Anti-ATP synthase subunit alpha antibodiesAS08 312 Anti-ATP synthase subunit gamma antibodiesAS05 071 Anti-ATP synthase subunit c antibodiesAS16 3976 Anti-AtpB Beta subunit of ATP synthase, mitochondrial, rabbit antibodiesPlant and algal protein extraction buffer Secondary antibodies
推荐的稀释浓度 :
1 : 100(IF), 1 : 5000(BN-PAGE), 1 : 2000-1 : 5 000(免疫印迹)
预计表观分子量 :
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
确认的物种反应活性 :
Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp. (roots, leafs, pollen), Nicotiana bentamiana, Nicotiana tabacum, Populus sp., Spinacia oleracea, Zea mays. Animal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria
前体活性 :
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp.
不反应 :
Archeal V-type ATP synthase
其他信息 :
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
选取的参考文献 :
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’) Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.Teng et al. (2013). Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase. PLOS ONE, June 2013. (immuofluorescence)Rasala et al. (2013). Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. March 23.Heinnickel et al. (2013). Novel thylakoid membrane greencut protein cpld38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes. J. Biol. Chem. Jan 9.
Pubmed登录号 :
28744300, 25617518, 25359543, 24043709, 23521393, 23303190
背景 :
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
重组 :
For reconstitution add 10 µl of sterile water.
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
This product can be sold containing proClin if requested.The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.
UniProt数据库数目 :
P19366, P83483, P06541, A8IQU3
TAIR数目 :
AT5G08670, ATCG00480
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。