产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
TOP2 DNA拓扑异构酶 II
目录 :
AS05 096
规格 :
200微升
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
拟南芥
应用 :
免疫印迹
更多信息或购买 :
产品信息
产品号 :
AS05 096
产品名称 :
TOP2 DNA拓扑异构酶 II
免疫原 :
The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
宿主 :
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
规格 :
200微升
检测过的应用 :
Immunolocalization (IL), Western blot (WB)
相关产品 :
AS07 225 Anti-A12.2 RNA polymerase I subunit (homolog of Pol II Rpb9), rabbit antibodiesAS07 265 Anti-eEF1b elongation factor eEF1b-beta protein, rabbit antibodiesPlant protein extraction buffer
推荐的稀释浓度 :
1 : 500(IL), 1 : 2000(免疫印迹)
预计表观分子量 :
164 170 kDa (Arabidopsis thaliana)
确认的物种反应活性 :
Arabidopsis thaliana, Vicia faba
前体活性 :
Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitcHensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox Caterii
不反应 :
烟草
其他信息 :
Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.This product can be sold containing ProClin if requested.
选取的参考文献 :
Xie S & Lam E (1994) Abundance of nuclear DNA topoisomerase II is correlated with proliferation in Arabidopsis thaliana. NAR 25:5729.Klaus Feldmann (1997) Regulation der Topoisomerase II von Arabidopsis thaliana im Zellzyklus. PhD thesis, University of Cologne.
Pubmed登录号 :
7846176
背景 :
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
重组 :
For reconstitution add 200 µl of sterile water.
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).
UniProt数据库数目 :
P30182
TAIR数目 :
AT3G23890
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。