产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
VTG Sole vitellogenin
目录 :
AS06 127
规格 :
200微升
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
产品号 :
AS06 127
产品名称 :
VTG Sole vitellogenin
免疫原 :
native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)
宿主 :
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
规格 :
200微升
检测过的应用 :
酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)
相关产品 :
collection of antibodies to fish proteinsSecondary antibodies
推荐的稀释浓度 :
1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)
预计表观分子量 :
ca. 200 kDa
确认的物种反应活性 :
Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
The developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.
选取的参考文献 :
Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.
Pubmed登录号 :
18342314
背景 :
Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.
重组 :
For reconstitution add 200 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
VTG can be purified using following methodology: Mañanós et al. (1994). Sea bass (Dicentrarchus labrax L.) vitellogenin. I—Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2): 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。