产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
H+ATPase血浆膜H+ATPase(兔抗体)
目录 :
AS07 260
规格 :
50微升
价格 :
302美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
red rice , 衣藻, 拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
文章摘录数: 21
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 衣藻; 1:2500; 图 s3b
Wang L, Yamano T, Takane S, Niikawa Y, Toyokawa C, Ozawa S, et al. Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016;113:12586-12591 pubmed
  • 免疫印迹; red rice ; 1:10,000; 图 s8
Huang X, Deng F, Yamaji N, Pinson S, Fujii Kashino M, Danku J, et al. A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. Nat Commun. 2016;7:12138 pubmed 出版商
  • 免疫印迹; 拟南芥; 1:1000; 图 1
Yang B, Han X, Yin L, Xing M, Xu Z, Xue H. Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016;7:11388 pubmed 出版商
  • 免疫印迹; 拟南芥; 图 1b
De Michele R, McFarlane H, Parsons H, Meents M, Lao J, González Fernández Niño S, et al. Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016;15:900-13 pubmed 出版商
Ekanayake G, Smith J, Jones K, Stiers H, Robinson S, LaMontagne E, et al. DYNAMIN-RELATED PROTEIN DRP1A functions with DRP2B in plant growth, flg22-immune responses, and endocytosis. Plant Physiol. 2021;: pubmed 出版商
Wang J, Chen T, Han M, Qian L, Li J, Wu M, et al. Plant NLR immune receptor Tm-22 activation requires NB-ARC domain-mediated self-association of CC domain. PLoS Pathog. 2020;16:e1008475 pubmed 出版商
Wang W, Liu N, Gao C, Cai H, Romeis T, Tang D. The Arabidopsis exocyst subunits EXO70B1 and EXO70B2 regulate FLS2 homeostasis at the plasma membrane. New Phytol. 2020;227:529-544 pubmed 出版商
Collins C, LaMontagne E, Anderson J, Ekanayake G, Clarke A, Bond L, et al. EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses. Plant Physiol. 2020;182:1762-1775 pubmed 出版商
Li X, Liu Q, Feng H, Deng J, Zhang R, Wen J, et al. Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy. 2019;:1-16 pubmed 出版商
Kuang L, Chen S, Guo Y, Ma H. Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019;10:641 pubmed 出版商
Yuan X, Wang Z, Huang J, Xuan H, Gao Z. Phospholipidase Dδ Negatively Regulates the Function of Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1). Front Plant Sci. 2018;9:1991 pubmed 出版商
Wang Q, Li Y, Ishikawa K, Kosami K, Uno K, Nagawa S, et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018;115:E11551-E11560 pubmed 出版商
Roth R, Chiapello M, Montero H, Gehrig P, Grossmann J, O Holleran K, et al. A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun. 2018;9:4677 pubmed 出版商
Deppe J, Rabbat R, Hortensteiner S, Keller B, Martinoia E, Lopez Marques R. The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane. J Biol Chem. 2018;293:18667-18679 pubmed 出版商
Pertl Obermeyer H, Lackner P, Schulze W, Hoepflinger M, Hoeftberger M, Foissner I, et al. Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS ONE. 2018;13:e0201480 pubmed 出版商
Deng F, Yamaji N, Ma J, Lee S, Jeon J, Martinoia E, et al. Engineering rice with lower grain arsenic. Plant Biotechnol J. 2018;16:1691-1699 pubmed 出版商
Nagel M, Kalinowska K, Vogel K, REYNOLDS G, Wu Z, Anzenberger F, et al. Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017;114:E7197-E7204 pubmed 出版商
Aloui A, Recorbet G, Lemaitre Guillier C, Mounier A, Balliau T, Zivy M, et al. The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis. Mycorrhiza. 2018;28:1-16 pubmed 出版商
Duan M, Zhang R, Zhu F, Zhang Z, Gou L, Wen J, et al. A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017;29:1748-1772 pubmed 出版商
Liao D, Cao Y, Sun X, Espinoza C, Nguyen C, Liang Y, et al. Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance. New Phytol. 2017;214:1646-1656 pubmed 出版商
Wattelet Boyer V, Brocard L, Jönsson K, Esnay N, Joubès J, Domergue F, et al. Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016;7:12788 pubmed 出版商
产品信息
产品号 :
AS07 260
产品名称 :
H+ATPase血浆膜H+ATPase(兔抗体)
规格 :
50微升
标价 :
302美元
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
免疫原 :
KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
宿主 :
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
检测过的应用 :
Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
推荐的稀释浓度 :
1 : 600-1 : 1000(IF), 1 : 100(IL), 1 : 1000-1 : 10 000(免疫印迹)
预计表观分子量 :
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
确认的物种反应活性 :
Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba
前体活性 :
Algae, Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis
不反应 :
Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba
其他信息 :
VERY IMPORTANT: Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents. This product can be sold containing ProClin if requested.
选取的参考文献 :
Michalopoulou et al. (2022) The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell. 2022 May 30:koac162. doi: 10.1093/plcell/koac162. Epub ahead of print. PMID: 35640532.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Huang et al (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell. 2021 Sep 30;184(20):5201-5214.e12. doi: 10.1016/j.cell.2021.08.029. Epub 2021 Sep 17. PMID: 34536345; PMCID: PMC8525514.Lapshin et al. (2021) Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects H+-ATPase Activity and H+-Transport. Biomolecules. 2021 Dec 16;11(12):1891. doi: 10.3390/biom11121891. PMID: 34944535; PMCID: PMC8699270.Choi et al. (2021) Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas. Nat Commun. 2021 Oct 18;12(1):6049. doi: 10.1038/s41467-021-26325-5. PMID: 34663809; PMCID: PMC8523702.
背景 :
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
重组 :
For reconstitution add 50 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.
其他信息 :
Cellular [compartment marker] for plasma membrane
UniProt数据库数目 :
P20649, P19456, P20431, Q9SU58, Q9SH76, Q9LY32, Q9M2A0, Q9M2A0, Q42556, Q9LV11, Q9FNS3
TAIR数目 :
AT2G18960, AT4G30190, AT5G57350, AT3G47950, AT2G07560, AT3G60330, AT3G42640, AT1G80660, AT5G62670
Commodity Code :
38220000
Our Category :
Arabidopsis thaliana
研究领域 :
植物生物学
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。