产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
H+ATPase血浆膜H+ATPase(兔抗体)
目录 :
AS07 260
规格 :
50微升
价格 :
302美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
red rice , 衣藻, 拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
文章摘录数: 21
出版应用/物种/样本/稀释 | 参考文献 |
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| Wang L, Yamano T, Takane S, Niikawa Y, Toyokawa C, Ozawa S, et al. Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016;113:12586-12591 pubmed
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产品信息
产品号 :
AS07 260
产品名称 :
H+ATPase血浆膜H+ATPase(兔抗体)
规格 :
50微升
标价 :
302美元
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
免疫原 :
KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
宿主 :
兔
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
检测过的应用 :
Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
推荐的稀释浓度 :
1 : 600-1 : 1000(IF), 1 : 100(IL), 1 : 1000-1 : 10 000(免疫印迹)
预计表观分子量 :
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
确认的物种反应活性 :
Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba
前体活性 :
Algae, Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis
不反应 :
Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba
其他信息 :
VERY IMPORTANT: Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents. This product can be sold containing ProClin if requested.
选取的参考文献 :
Michalopoulou et al. (2022) The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell. 2022 May 30:koac162. doi: 10.1093/plcell/koac162. Epub ahead of print. PMID: 35640532.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Huang et al (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell. 2021 Sep 30;184(20):5201-5214.e12. doi: 10.1016/j.cell.2021.08.029. Epub 2021 Sep 17. PMID: 34536345; PMCID: PMC8525514.Lapshin et al. (2021) Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects H+-ATPase Activity and H+-Transport. Biomolecules. 2021 Dec 16;11(12):1891. doi: 10.3390/biom11121891. PMID: 34944535; PMCID: PMC8699270.Choi et al. (2021) Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas. Nat Commun. 2021 Oct 18;12(1):6049. doi: 10.1038/s41467-021-26325-5. PMID: 34663809; PMCID: PMC8523702.
背景 :
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
重组 :
For reconstitution add 50 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.
其他信息 :
Cellular [compartment marker] for plasma membrane
UniProt数据库数目 :
P20649, P19456, P20431, Q9SU58, Q9SH76, Q9LY32, Q9M2A0, Q9M2A0, Q42556, Q9LV11, Q9FNS3
TAIR数目 :
AT2G18960, AT4G30190, AT5G57350, AT3G47950, AT2G07560, AT3G60330, AT3G42640, AT1G80660, AT5G62670
Commodity Code :
38220000
Our Category :
Arabidopsis thaliana
研究领域 :
植物生物学
更多信息或购买 :
公司信息
Agrisera
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。