产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
木糖
目录 :
AS07 267
规格 :
50微克
价格 :
485美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹
更多信息或购买 :
Agrisera 产品网页
文章摘录数: 6
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 拟南芥; 1:10,000; 图 s2
Rautengarten C, Ebert B, Liu L, Stonebloom S, Smith Moritz A, Pauly M, et al. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development. Nat Commun. 2016;7:12119 pubmed 出版商
Yang C, Wang C, Singh S, Fan N, Liu S, Zhao L, et al. Golgi-localised manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytol. 2021;: pubmed 出版商
Lucas P, Mathieu Rivet E, Song P, Oltmanns A, Loutelier Bourhis C, Plasson C, et al. Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2020;102:230-245 pubmed 出版商
Jansing J, Sack M, Augustine S, Fischer R, Bortesi L. CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose. Plant Biotechnol J. 2019;17:350-361 pubmed 出版商
Nakanishi K, Morikane S, Ichikawa S, Kurohane K, Niwa Y, Akimoto Y, et al. Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA. Sci Rep. 2017;7:45843 pubmed 出版商
Hanania U, Ariel T, Tekoah Y, Fux L, Sheva M, Gubbay Y, et al. Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins. Plant Biotechnol J. 2017;15:1120-1129 pubmed 出版商
产品信息
产品号 :
AS07 267
产品名称 :
木糖
规格 :
50微克
标价 :
485美元
免疫原 :
Xylose residues bound to the N-glycan in beta 1,2
宿主 :
兔
克隆性 :
多克隆
纯度 :
免疫原亲和纯化血清在PBS pH值7.4
格式 :
冻干
检测过的应用 :
免疫印迹(免疫印迹)
推荐的稀释浓度 :
1微克/毫升(酶联免疫吸附测定), 2微克/10毫升培养缓冲液(免疫印迹)
预计表观分子量 :
10-100 for various glycoproteins
确认的物种反应活性 :
Higher plants and algae
前体活性 :
Higher plants
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250
相关产品 :
Plant and algal protein extraction buffer Secondary antibodies
选取的参考文献 :
Xavier et al. (2021) Inactivation of N-Acetylglucosaminyltransferase I and a1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans. Frontiers in Plant Science. Volume 12. doi: 10.3389/fpls.2021.634023Yang et al. (2021). Golgi-localized manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytol. 2021 Jan 17. doi: 10.1111/nph.17209. Epub ahead of print. PMID: 33454966.Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620. Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620.Hou et al. (2019). Identification and characterization of a novel glycoprotein core xylosidase from the bacterium Elizabethkingia meningoseptica. Biochemical and Biophysical Research Communications Available online 27 July 2019.
背景 :
This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
重组 :
For reconstitution add 50 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.
Commodity Code :
38220000
Our Category :
碳水化合物
研究领域 :
植物生物学
更多信息或购买 :
Agrisera 产品网页
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。