抗体

HSP101 ClpB heat shock protein, C-terminal

AS08 287

50微升

多克隆

兔

nonconjugated

HSP101 ClpB heat shock protein, C-terminal

Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids P42730, At1g74310

兔

多克隆

血清

冻干

50微升

免疫印迹(免疫印迹)

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1 : 1000(免疫印迹)

101 kDa

Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak

Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera

No confirmed exceptions from predicted reactivity are currently known.

When testing a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

Balfagón et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029. Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.

29609175, 23499455, 22451724

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

P42730

AT1G74310