V-ATPase, a Vacuolar H+ ATPase, subunit a | Agrisera AS09 466 产品信息

产品简要

公司名称 :

Agrisera

产品类型 :

抗体

产品名称 :

V-ATPase, a Vacuolar H+ ATPase, subunit a

AS09 466

规格 :

100微升

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

nonconjugated

反应物种 :

拟南芥

应用 :

免疫印迹, 酶联免疫吸附测定

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产品信息

产品号 :

AS09 466

产品名称 :

V-ATPase, a Vacuolar H+ ATPase, subunit a

免疫原 :

KLH-cougted synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit a, Q9SJT7, At2g21410

宿主 :

兔

克隆性 :

多克隆

纯度 :

血清

格式 :

冻干

规格 :

100微升

检测过的应用 :

酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)

相关产品 :

collection of antibodies to vacuolar proteins AS09 467 anti-V-ATPase subunit A vacuolar H+-ATPaseAS09 503 anti-V-ATPase, B vacuolar ATP synthase subunit betaAS09 468 anti-V-ATPase subunit c vacuolar H+-ATPase, subunit c (16 kDa)AS09 497 anti- V-ATPase subunit D V-type proton ATPase subunit DAS07 213 anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 anti-V-ATPase subunit H V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies

推荐的稀释浓度 :

1 : 8000(酶联免疫吸附测定), 1 : 2000(免疫印迹)

预计表观分子量 :

93 100 kDa (Arabidopsis thaliana)

确认的物种反应活性 :

Arabidopsis thaliana, Cucumis sativus, Oryza sativa

前体活性 :

Chlamydomonas reainhardtii, Physcomitrella patens, Populus balsamifera, Ricinus communis, Vitis vinifera

不反应 :

No confirmed exceptions from predicted reactivity are currently known.

其他信息 :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.

选取的参考文献 :

Xing et al. (2016). Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS One. 2016 Dec 19;11(12):e0168467. doi: 10.1371/journal.pone.0168467. Migocka et al. (2013). NO3 (-)/H(+) Antiport in the Tonoplast of Cucumber Root Cells Is Stimulated by Nitrate Supply: Evidence for a Reversible Nitrate-Induced Phosphorylation of Vacuolar NO3 (-)/H(+) Antiport.PLoS One. 2013 Sep 11;8(9):e73972. doi: 10.1371/journal.pone.0073972.Fumiyoshi et al. (2005). Novel type aquaporin SIPs, are mainly localized the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579: 5814-58200.Yoshihiro et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 45: 1749-1758.

Pubmed登录号 :

27992503, 24040130, 16223486, 15653794

背景 :

V-ATPase subunit a is coded by VHA-A2 gene. It has hydrogen ion transmembrane transporter activity. Alternative names: At2g21410/F3K23.17, putative vacuolar proton-ATPase subunit, V-ATPase subunit a (100 kDa subunit), VHA-a

重组 :

For reconstitution add 100 µl of sterile water.

储存 :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

其他信息 :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar membranes are used, one µg load per well should be sufficient.Protocol of isolation of plant vacuolar membranes can be found here.

UniProt数据库数目 :

Q9SJT7

TAIR数目 :

AT2G21410

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