V-ATPase, A Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

产品简要

公司名称 :

Agrisera

产品类型 :

抗体

产品名称 :

V-ATPase, A Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

AS09 467

规格 :

100微升

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

nonconjugated

反应物种 :

拟南芥

应用 :

免疫印迹, 酶联免疫吸附测定

更多信息或购买 :

Agrisera 产品网页

文章摘录数: 1

参考文献
Vera Estrella R, Barkla B, Pantoja O. Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014;111:113-27 pubmed 出版商

产品信息

产品号 :

AS09 467

产品名称 :

V-ATPase, A Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

免疫原 :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900

宿主 :

兔

克隆性 :

多克隆

纯度 :

硫酸铵纯化IgG

格式 :

冻干

规格 :

100微升

检测过的应用 :

酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)

相关产品 :

Collection of antibodies to other vacuolar membrane proteinsPlant protein extraction bufferSecondary antibodies

推荐的稀释浓度 :

1 : 8000(酶联免疫吸附测定), 1 : 2000(免疫印迹)

预计表观分子量 :

68.8 70 kDa (Arabidopsis thaliana)

确认的物种反应活性 :

Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum

前体活性 :

Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrella patens, Solanum lycopersicon, Triticum aestivum, Zea mays

不反应 :

No confirmed exceptions from predicted reactivity are currently known.

其他信息 :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

选取的参考文献 :

Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1. Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.Yoshihiro et al. (2006) Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells. Cells. Biosci.Biotechnol. Biochem. 70: 980-987.

Pubmed登录号 :

28455771, 27160145, 16636467

背景 :

V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

重组 :

For reconstitution add 100 µl of sterile water.

储存 :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

其他信息 :

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plant vacuolar membranes can be found here.

UniProt数据库数目 :

O23654

TAIR数目 :

AT1G78900

更多信息或购买 :

Agrisera 产品网页

公司信息