V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa)

产品简要

公司名称 :

Agrisera

产品类型 :

抗体

产品名称 :

V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa)

AS09 468

规格 :

50微克

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

nonconjugated

反应物种 :

拟南芥

应用 :

免疫印迹

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产品信息

产品号 :

AS09 468

产品名称 :

V-ATPase, c Vacuolar H+-ATPase, subunit c (16 kDa)

免疫原 :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c UniProt: Q6IDA4

宿主 :

兔

克隆性 :

多克隆

纯度 :

亲和纯化血清在PBS, pH值7.4

格式 :

冻干

规格 :

50微克

检测过的应用 :

免疫印迹(免疫印迹)

相关产品 :

Collection of antibodies to vacuolar proteins AS09 467 anti-V-ATPase subunit A vacuolar H+-ATPaseAS09 503 anti-V-ATPase, B vacuolar ATP synthase subunit betaASS09 497 anti- V-ATPase subunit D V-type proton ATPase subunit DAS07 213 anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 anti-V-ATPase subunit H V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies

推荐的稀释浓度 :

1 : 1000(免疫印迹)

预计表观分子量 :

16 16 kDa (Arabidopsis thaliana)

确认的物种反应活性 :

Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum

前体活性 :

dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa

不反应 :

藻类

其他信息 :

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

选取的参考文献 :

Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1. Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.

Pubmed登录号 :

28455771, 27160145

背景 :

V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300

重组 :

For reconstitution add 50 µl of sterile water.

储存 :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

其他信息 :

Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min.

UniProt数据库数目 :

Q6IDA4

TAIR数目 :

AT2G16510

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