产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
BiP Lumenal-binding protein (rabbit antibody)
目录 :
AS09 481
规格 :
50微克
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 拟南芥; 1:2000; 图 4
Jozwiak A, Gutkowska M, Gawarecka K, Surmacz L, Buczkowska A, Lichocka M, et al. POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility. Plant Cell. 2015;27:3336-53 pubmed 出版商
  • 免疫印迹; scFv; 1:2000; 图 7a
Wilson S, Ho Y, Lampugnani E, van de Meene A, Bain M, Bacic A, et al. Determining the subcellular location of synthesis and assembly of the cell wall polysaccharide (1,3; 1,4)-β-D-glucan in grasses. Plant Cell. 2015;27:754-71 pubmed 出版商
Wang Q, Li Y, Ishikawa K, Kosami K, Uno K, Nagawa S, et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018;115:E11551-E11560 pubmed 出版商
Mares J, Gramacho K, Santos E, da Silva Santiago A, Santana J, de Sousa A, et al. Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017;17:176 pubmed 出版商
Buxa S, Degola F, Polizzotto R, De Marco F, Loschi A, Kogel K, et al. Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. Front Plant Sci. 2015;6:650 pubmed 出版商
Lopez Huertas E, del Rio L. Characterization of antioxidant enzymes and peroxisomes of olive (Olea europaea L.) fruits. J Plant Physiol. 2014;171:1463-71 pubmed 出版商
Tanaka H, Nodzyłski T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, et al. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis. Plant Cell Physiol. 2014;55:737-49 pubmed 出版商
Sikorskaite S, Rajamäki M, Baniulis D, Stanys V, Valkonen J. Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families. Plant Methods. 2013;9:31 pubmed 出版商
Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, et al. Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis thaliana. PLoS Genet. 2013;9:e1003540 pubmed 出版商
产品信息
产品号 :
AS09 481
产品名称 :
BiP Lumenal-binding protein (rabbit antibody)
免疫原 :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3
宿主 :
克隆性 :
多克隆
纯度 :
亲和纯化血清在PBS, pH值7.4
格式 :
冻干
规格 :
50微克
检测过的应用 :
ELISA (ELISA), Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
相关产品 :
AS09 615 BiP2 lumenal-binding protein 2, goat antibodyAS09 614 BiP2 lumenal-binding protein 2, hen antibodyAS09 481PRE BiP lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
推荐的稀释浓度 :
1 : 8000(酶联免疫吸附测定), 1 : 600(IF), 1 : 2000(免疫印迹)
预计表观分子量 :
73.5 80 kDa
确认的物种反应活性 :
Arabidopsis thaliana, Brassica napus, Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Physcomitrella patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
前体活性 :
Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Prunus persica, Triticum aestivium, Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis vinifera
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.
选取的参考文献 :
Pertl-Obermeyer et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480. Qiao et al. (2018). Two Crinivirus-Conserved Small Proteins, P5 and P9, Are Indispensable for Efficient Lettuce infectious yellows virus Infectivity in Plants. Viruses. 2018 Aug 28;10(9). pii: E459. doi: 10.3390/v10090459. Mares et al. (2017). Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches' broom disease in cacao. BMC Microbiol. 2017 Aug 17;17(1):176. doi: 10.1186/s12866-017-1085-4. Gelová et al. (2017). Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. J Exp Bot. 2017 Dec 23. doi: 10.1093/jxb/erx426.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114. Lomin et al. (2017). Studies of cytokinin receptor–phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. Functional Plant Biology, CSIRO Publications. (Nicotiana benthamiana, western blot) Zhang et al. (2017). Control of secondary cell wall patterning involves xylan deacetylation by a GDSL esterase. Nat Plants. 2017 Mar 3;3:17017. doi: 10.1038/nplants.2017.17. (Oryza sativa, immunolocalization, western blot)Je et al. (2016). Signaling from maize organ primordia via FASCIATED EAR3 regulates stem cell proliferation and yield traits. Nat Genet. 2016 Jul;48(7):785-91. doi: 10.1038/ng.3567. Epub 2016 May 16.
Pubmed登录号 :
30157181, 30154314, 28818052, 29294075, 28784794, 28260782, 27182966
背景 :
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
重组 :
For reconstitution add 50 µl of sterile water.
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt数据库数目 :
Q39043
TAIR数目 :
AT5G42020, AT5G42020
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
https://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。