抗体

PIP2;1+PIP2;2水通道蛋白PIP2;1+PIP2;2

AS09 488

100微升

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定

PIP2;1+PIP2;2水通道蛋白PIP2;1+PIP2;2

KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2 UniProt:O80369

兔

多克隆

血清

冻干

100微升

酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)

collection of antibodies to plasma membrane proteins AS09 489 Anti-PIP1;1, PIP1;2, PIP1;3 aquaporins, rabbit antibodiesAS09 490 Anti-PIP2;2 plasma membrane aquaporin 2b, rabbit antibodiesAS09 491 Anti-PIP2;1,PIP2;2,PIP2;3 plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies

1 : 8000(酶联免疫吸附测定), 1 : 1000(免疫印迹)

30.34 28 kDa (Raphanus sativus)

Arabidopsis thaliana, Oryza sativa, Raphanus sativus

Brassica napus, Gossypium mexicanumSpecies of your interest not listed? Contact us

Hordeum vulgare, Vitis vinifera

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Peptide used to elicit this antibody is also conserved in Arabidopsis thaliana PIP2;1,PIP2;2, PIP2;3 and this antibody reacted also with PIP2;2 in experiments with knock out plants.

Markus G. et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane.

For reconstitution add 100 µl of sterile water

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

O80369

AT3G53420

Arabidopsis thaliana