产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
PIP2;2血浆膜水通道蛋白2b
目录 :
AS09 490
规格 :
100微升
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
产品号 :
AS09 490
产品名称 :
PIP2;2血浆膜水通道蛋白2b
免疫原 :
KLH-conjugated synthetic peptide derived from N-terminal part of Raphanus sativus PAQ2b UniProt: Q9MB99
宿主 :
兔
克隆性 :
多克隆
纯度 :
血清
格式 :
冻干
规格 :
100微升
检测过的应用 :
酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)
相关产品 :
collection of antibodies to plasma membrane proteins AS09 488 Anti-PIP2;1 aquaporin PIP2;1, rabbit antibodiesAS09 489 Anti-PIP1;1, PIP1;2, PIP1;3 aquaporins, rabbit antibodiesAS09 491 Anti-PIP2;1,PIP2;2,PIP2;3 plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
推荐的稀释浓度 :
1 : 8000(酶联免疫吸附测定), 1 : 2000(免疫印迹)
预计表观分子量 :
30 28 kDa (Raphanus sativus)
确认的物种反应活性 :
Arabidopsis thaliana, Fragaria ananassa, Raphanus sativus
前体活性 :
Brassica sp. Species of your interest not listed? Contact us
不反应 :
No confirmed exceptions from predicted reactivity are currently known
其他信息 :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antiboy will weakly react with PIP2;1 and theoretically with PIP2;3, however this protein is very scarcely expressed in plant tissues. The mRNA content is 1 % of that for PIP2;2, therefore reactivity to PIP2;3 was neglegible in our experiments.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
选取的参考文献 :
Brillada et al. (2020). Exocyst subunit Exo70B2 is linked to immune signalling and autophagy, The Plant Cell, 2020;, koaa022, https://doi.org/10.1093/plcell/koaa022
背景 :
PIP2;2 is a plasma membrane aquaporin.
重组 :
For reconstitution add 100 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
UniProt数据库数目 :
Q9MB99
TAIR数目 :
AT2G37170
研究领域 :
Arabidopsis thaliana
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公司信息
Agrisera
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。