抗体

PIP2;3 Aquaporin, plasma membrane intrinistic protein 2-3

AS09 506

100微升

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定

PIP2;3 Aquaporin, plasma membrane intrinistic protein 2-3

KLH-conjugated peptide derived from Oryza sativa PIP2;3, UniProt: Q7XUA6

兔

多克隆

血清

冻干

100微升

Chromogenic Immunohistochemistry (CIH), ELISA (ELISA), Immunogold (IG), Western blot (WB)

collection of antibodies to plasma membrane proteins AS09 505 Anti-PIP1s aquaporin, rabbit antibodiesAS09 507 Anti-PIP2;1 aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 508 Anti-PIP2;5 aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodiesPlant protein extraction buffer

1: 50(CIH), 1 : 8000(酶联免疫吸附测定), 1 : 100(IgE), 1 : 2000(免疫印迹)

31,9 26-29 kDa

水稻, 玉米

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Arabidopsis thaliana, Hordeum vulgare, Solanum tuberosum

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3

For reconstitution add 100 µl of sterile water

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. This antibody will recognize PIP2;3 in root tissue only. No signal is obtained for leaf tissue. For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

Q6Z2T3

水稻