抗体

V-ATPase Epsilon subunit of tonoplast H+ATPase (goat antibody)

AS09 577

300微升

394美元

多克隆

domestic goat

未共轭

V-ATPase Epsilon subunit of tonoplast H+ATPase (goat antibody)

300微升

394美元

KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

山羊

多克隆

血清

冻干

免疫印迹(免疫印迹)

1 : 1000-1 : 3000(免疫印迹)

26 31 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum

Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. , Physcomitrium patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat

No confirmed exceptions from predicted reactivity are currently known.

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Plant protein extraction bufferSecondary antibodies

McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.

Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

For reconstitution add 300 µl of sterile water

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Q39258

AT4G11150

38220000

Vacuolar membrane

植物生物学