产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
微管蛋白乙链
目录 :
AS10 681
规格 :
100微克
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
文章摘录数: 3
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产品信息
产品号 :
AS10 681
产品名称 :
微管蛋白乙链
免疫原 :
KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890).
宿主 :
兔
克隆性 :
多克隆
纯度 :
免疫原亲和纯化血清在PBS pH值7.4
格式 :
冻干
规格 :
100微克
检测过的应用 :
免疫荧光(IF), 免疫印迹(免疫印迹)
相关产品 :
AS10 680 Anti-tubulin alpha chain, rabbit antibodiesPlant and algal protein extraction buffer
推荐的稀释浓度 :
1 : 1000(IF), 1 : 500(免疫印迹)
预计表观分子量 :
49 49 kDa (Arabidopsis thaliana)
确认的物种反应活性 :
Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
前体活性 :
Brassica napus, Glycine max, Hordeum vulgare, Micromonoas pusilla, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrium patens, Populus trichocarpa, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us
不反应 :
No confirmed exceptions from predicted reactivity are currently known
其他信息 :
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.
选取的参考文献 :
Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
背景 :
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
重组 :
For reconstitution add 100 µl of sterile water
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
其他信息 :
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.
UniProt数据库数目 :
P29512, P24636, P29513, P29514, P29515, P29516, P29517
TAIR数目 :
AT5G62690
研究领域 :
Arabidopsis thaliana
更多信息或购买 :
公司信息
Agrisera
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。