产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
P-Tyr Phosphotyrosine
目录 :
AS10 705
规格 :
100微克
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
IgG1(克隆G104)
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
产品信息
产品号 :
AS10 705
产品名称 :
P-Tyr Phosphotyrosine
免疫原 :
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide
宿主 :
小鼠
克隆性 :
单克隆
克隆编号 :
IgG1(克隆G104)
纯度 :
蛋白质G纯化
格式 :
Liquid
规格 :
100微克
检测过的应用 :
免疫沉淀(免疫沉淀), 免疫荧光(IF), 免疫组化(免疫组化), 免疫印迹(免疫印迹)
相关产品 :
Plant and algal protein extraction buffer Secondary antibodies
推荐的稀释浓度 :
1 : 1000(免疫印迹)
确认的物种反应活性 :
Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
选取的参考文献 :
Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF): Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
Pubmed登录号 :
8887669, 10488121, 9920935
背景 :
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
储存 :
Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
Protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml.
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。