抗体

Amyloid beta oligomer-specific monoclonal antibody (OMAB)

AS10 932

50微克

单克隆

小鼠

nonconjugated

IgM

酶联免疫吸附测定, 免疫组化

Amyloid beta oligomer-specific monoclonal antibody (OMAB)

partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V. The epitope is 3-8. Molecular weight of immunogen is 4.5 kDa.

小鼠

单克隆

IgM

亲和纯化

冻干

50微克

酶联免疫吸附测定(酶联免疫吸附测定), 免疫组化(免疫组化)

AS13 2716 mAB-M human Abeta protein (3-10) region, oligomer-specific, mouse monoclonal antibodyAS13 2715 mAB-O human Abeta protein (3-10) region, oligomer specific, mouse monoclonal antibodyAgrisera matching secondary antibody: Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum, AS10 969Secondary antibodies

Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)

4.5 kDa

Human Abeta oligomers only

大鼠

No confirmed exceptions from predicted reactivity are currently known.

OMAB antibody is a versatile tool within research of Alzheimer’s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Abeta oligomers.

Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of Abeta Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401. Kumar et al. (2017). Foldamer-Mediated Structural Rearrangement Attenuates Abeta Oligomerization and Cytotoxicity. J Am Chem Soc. 2017 Nov 29;139(47):17098-17108. doi: 10.1021/jacs.7b08259. Zhao et al. (2016). Antiamyloidogenic Activity of Abeta42-Binding Peptoid in Modulating Amyloid Oligomerization. Small. 2016 Oct 7. doi: 10.1002/smll.201602857.Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.Lindhagen-Persson et al. (2010). Amyloid-beta Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.

29648815, 29058422, 27714968, 21085663

Soluble oligomeric assemblies of the Amyloid-beta peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Abeta-assemblies constitute a very interesting therapeutic target. Identification of Abeta-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Abeta-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

For reconstitution add 100 µl of sterile water.

Store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.