抗体

FtsH1-11 ATP-dependent zinc metalloprotease FtsH1-11

AS11 1789

50微升

多克隆

兔

nonconjugated

FtsH1-11 ATP-dependent zinc metalloprotease FtsH1-11

KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1

兔

多克隆

血清

冻干

50微升

Blue Native PAGE (BN-PAGE), Western blot (WB)

AS11 1789S FtsH2 positive control/quantitation standardAS16 3930 anti-FtsH1 + FtsH5 ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic)AS16 3929 anti-FtsH2 + FtsH8 ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic)AS07 204 anti-FtsH3 + FtsH10 ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)AS07 205 anti-FtsH4 ATP-dependent zinc metalloprotease FtsH4 (mitochondrial)AS05 094A anti-FtsH6 ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)AS06 130 anti-FtsH9 ATP-dependent zinc metalloprotease FtsH9 (chloroplastic)AS07 251 anti-FtsH10 ATP-dependent zinc metalloprotease FtsH10 (mitochondrial)Antibodies to other proteins involved in photosynthesisPlant protein extraction bufferSecondary antibodies

1 : 500-1 : 2000(免疫印迹)

A. thaliana: FtsH1:76.7, FtsH2: 74, FtsH5: 75.2, FtsH6: 74.5, FtsH7: 87.8, FtsH8: 73, FtsH9:87.8 kDa (chloroplastic); FtsH3: 89.3, FtsH4: 77.2, FtsH10: 89.5 kDa, FtsH11:88.7 kDa (mitochondrial)

Acetabularia acetabulum, Arabidopsis thaliana, Bryopsis hypnoides (ulvophytes), Guillardia theta, Hordeum vulgare, Synechocystis sp., Thalassiosira pseudonana, Thalassiosira punctigera, Zea mays

Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots

No confirmed exceptions from predicted reactivity are currently known.

For detection on a diatom samples, load of 5-10 µg/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.

Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Tietz et al. (2015). Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes. J Biol Chem. 2015 Apr 20. pii: jbc.M114.619841.Campbell et al. (2013). Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana. Photosynth. Res. March 16.

25897076, 23504483

FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Q84WU8, Q8VZI8

AT1G50250, AT2G29080