BRI1 Brassinosteroid insensitive 1 | Agrisera AS12 1859 产品信息

产品简要

公司名称 :

Agrisera

产品类型 :

抗体

产品名称 :

BRI1 Brassinosteroid insensitive 1

AS12 1859

规格 :

50微克

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

nonconjugated

反应物种 :

拟南芥

应用 :

免疫印迹, 免疫沉淀

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产品信息

产品号 :

AS12 1859

产品名称 :

BRI1 Brassinosteroid insensitive 1

免疫原 :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400

宿主 :

兔

克隆性 :

多克隆

纯度 :

亲和纯化血清在PBS, pH值7.4

格式 :

冻干在PBS pH值7.4

规格 :

50微克

检测过的应用 :

免疫沉淀(免疫沉淀), 免疫印迹(免疫印迹)

相关产品 :

AS12 1858 anti-BAK1 Brassinosteroid insensitive 1-associated receptor kinase 1, rabbit antibodiesAS16 3203 Anti-BIN2 brassinosteroid insensitive 2 antibodyAS16 3204 Anti-SOBIR1 supressor of BIR1 antibodyPlant protein extraction buffer Secondary antibodies

推荐的稀释浓度 :

1 : 5000(免疫印迹)

预计表观分子量 :

above 130 kDa (due to N-glycosylation)

确认的物种反应活性 :

Arabidopsis thaliana

前体活性 :

芜菁

不反应 :

大麦, 水稻

其他信息 :

Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.

选取的参考文献 :

to be added when available, antibody released in July 2014.

背景 :

BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase

重组 :

For reconstitution add 50 µl of sterile water.

储存 :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt数据库数目 :

O22476

TAIR数目 :

AT4G39400

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