产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
ABI1 Abscisic acid insensitive 1
目录 :
AS12 1861
规格 :
50微克
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样品/稀释参考文献
  • 免疫印迹; 拟南芥; 图 7
Mitula F, Tajdel M, CieÅ›la A, Kasprowicz MaluÅ›ki A, Kulik A, Babula SkowroÅ„ska D, et al. Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015;56:2351-67 pubmed 出版商
产品信息
产品号 :
AS12 1861
产品名称 :
ABI1 Abscisic acid insensitive 1
免疫原 :
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
宿主 :
克隆性 :
多克隆
纯度 :
亲和纯化血清在PBS pH值7.4
格式 :
冻干
规格 :
50微克
检测过的应用 :
免疫沉淀(免疫沉淀), 免疫印迹(免疫印迹)
相关产品 :
AS12 1871 ABI2 abscisic acid insensitive 2, rabbit antibodyAS12 1862 ABI3 abscisic acid insensitive 3, rabbit antibodyAS12 1863 ABI5 abscisic acid insensitive 5, rabbit antibodyAS13 2634 PYR1 Abscisic acid receptor RCAR11, rabbit antibodyAS13 2635 SRK2E Ser/Thr-protein kinase SnRK2.6, rabbit antibodyPlant protein extraction buffer Secondary antibodies
推荐的稀释浓度 :
5 µg (IP for a 200 ul of a cell extract), 3 µg (WB)
预计表观分子量 :
47.5 kDa
确认的物种反应活性 :
Arabidopsis thaliana
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.
选取的参考文献 :
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Pubmed登录号 :
26443375
背景 :
ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.
重组 :
For reconstitution add 50 µl of sterile water.
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal conditions. The antibody detects both, recombinant and endogenous ABI1 protein.
UniProt数据库数目 :
P49597
TAIR数目 :
AT4G26080
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。