产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
DCL1 Dicer-like protein 1
目录 :
AS12 2102
规格 :
100微克
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
拟南芥
应用 :
免疫印迹, 免疫沉淀, 核糖核酸免疫沉淀
更多信息或购买 :
文章摘录数: 1
产品信息
产品号 :
AS12 2102
产品名称 :
DCL1 Dicer-like protein 1
免疫原 :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL1 sequence, Q9SP32 , At1g01040
宿主 :
兔
克隆性 :
多克隆
纯度 :
亲和纯化血清在PBS, pH值7.4
格式 :
冻干
规格 :
100微克
检测过的应用 :
RIP (RNA Immunoprecipitation (IP) assay), Western blot (WB)
相关产品 :
AS15 3100 anti-DCL2 Dicer-like protein 2, rabbit antibodies AS12 2103 anti-DCL3 Dicer-like protein 3, rabbit antibodiesAS15 3105 anti-DCL4 Dicer-like protein 4Plant protein extraction buffer Secondary antibodies
推荐的稀释浓度 :
1 : 1000(免疫印迹)
预计表观分子量 :
213 kDa
确认的物种反应活性 :
Arabidopsis thaliana
前体活性 :
Arabidopsis thaliana
不反应 :
玉米
其他信息 :
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
选取的参考文献 :
Su et al. (2017). The Protein Phosphatase 4 and SMEK1 Complex Dephosphorylates HYL1 to Promote miRNA Biogenesis by Antagonizing the MAPK Cascade in Arabidopsis. Dev Cell. 2017 Jun 5;41(5):527-539.e5. doi: 10.1016/j.devcel.2017.05.008. Yoshikawa (2016). Pre-microRNA processing activity in nuclear extracts from Arabidopsis suspension cells. J Plant Res. 2016 Nov 24. [Epub ahead of print]Li et al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.Francisco-Mangilet et al. (2015). THO2, core member of the THO/TREX complex, is required for micro RNA production in Arabidopsis. Plant J. 2015 May 14. doi: 10.1111/tpj.12874.
Pubmed登录号 :
28586645, 27885505, 27870853, 25976549
背景 :
DCL1 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Protein ABNORMAL SUSPENSOR 1, Protein CARPEL FACTORY, Protein SHORT, INTEGUMENTS 1, Protein SUSPENSOR 1.
重组 :
For reconstitution add 50 µl of sterile water.
储存 :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
其他信息 :
Freezing and re-using of diluted anti-DCL1 antibodies is not recommended and will contribute to non-conclusive results.
UniProt数据库数目 :
Q9SP32
TAIR数目 :
AT1G01040
更多信息或购买 :
公司信息
Agrisera
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。