产品简要
公司名称 :
Agrisera
产品类型 :
抗体
产品名称 :
TNFa肿瘤坏死因子α, 生物素共轭标签
目录 :
IMS06-102-325
规格 :
100微升
克隆性 :
多克隆
宿主 :
共轭标签 :
生物素
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
产品号 :
IMS06-102-325
产品名称 :
TNFa肿瘤坏死因子α, 生物素共轭标签
免疫原 :
纯化全长重组蛋白P01375
宿主 :
克隆性 :
多克隆
纯度 :
亲和纯化IgY
格式 :
液体0.15M氯化钠, 0.02M钠磷酸盐, 0.1% 叠氮化钠, pH值7.2
规格 :
100微升
检测过的应用 :
酶联免疫吸附测定(酶联免疫吸附测定), 免疫印迹(免疫印迹)
相关产品 :
IMS01-102-339 anti-TNFa Tumor necrosis factor alpha, chicken antibodyIMS01-142-310 anti-Etanercept (Enbrel®), recombinant human tumor necrosis factor receptor, chicken antibody
推荐的稀释浓度 :
1 : 5000(免疫印迹)
预计表观分子量 :
26 kDa
确认的物种反应活性 :
人类
前体活性 :
猫, 狗, 马, 猪, 灵长类动物
不反应 :
No confirmed exceptions from predicted reactivity are currently known.
其他信息 :
The antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots.
选取的参考文献 :
to be added when available
背景 :
Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
储存 :
Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
其他信息 :
The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin.Total IgY concentration for this antibody is 15 mg/ml.
UniProt数据库数目 :
P01375
更多信息或购买 :
公司信息
Agrisera
Box 57
911 21 Vannas
support@agrisera.com
http://www.agrisera.com
46-0-935-33000
公司总部: 瑞典
Agrisera是一家专业生产多克隆和单克隆抗体瑞典公司。高质量的服务水平和确实有效的抗体。30年来在与世界各地的制药公司和研究团体密切合作。