抗体

Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody

AAR-011-AO

1 X 50 mcl

735美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫组化, 免疫细胞化学

AAR-011-AO

Primary Antibody>Extracellular>Labeled

Insert

Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications, and will recognize AT1 receptor from rat, mouse and human samples. nAnti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550

BLP-AR011

血管紧张素Ⅱ受体类型1, AT1受体, AT1R, AGTR1

血管紧张素Ⅱ受体类型1

G-Protein Coupled Receptors > Angiotensin II Receptors > Antibodies

ATTO 550 (Orange)

免疫细胞化学, IF, 免疫组化, LCI

兔

多克隆

兔IgG

AGTR1

人类(试验);小鼠(试验);大鼠(试验)

Mouse, rat, rabbit, dog - identical.

Angiotensin II receptor type 1 (AT1, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

合成肽

细胞外

细胞外, N末端

NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1

Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 (Accession P30556). Extracellular, N-terminus.

1 X 50 mcl

1毫克/毫升

1毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P30556

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

735美元

Expression of Angiotensin II Receptor Type-1 in rat C6 glioma cells - Cell surface detection of Angiotensin II Receptor Type-1 in live intact rat C6 glioma cells with Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO), (1:50), (left panel). Right panel shows live view of the same field.