抗体

抗血管紧张素Ⅱ受体类型1(细胞外)-FITC抗体

AAR-011-F

1 X 50 mcl

735美元

多克隆

domestic rabbit

异硫氰酸荧光素

人类, 小鼠, 大鼠

AAR-011-F

Primary Antibody>Extracellular>Labeled

Insert

抗血管紧张素Ⅱ受体类型1(细胞外)-FITC抗体

Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples. nAnti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye FITC

RIC-001-F

血管紧张素Ⅱ受体类型1, AT1受体, AT1R, AGTR1

血管紧张素Ⅱ受体类型1

G-Protein Coupled Receptors > Angiotensin II Receptors > Antibodies

荧光素异硫氰酸酯(异硫氰酸荧光素)

FC, LCI

兔

多克隆

兔IgG

AGTR1

人类(试验);小鼠(试验);大鼠(试验)

Mouse, rat, rabbit, dog - identical.

The biological functions of angiotensin II are mediated by AT1 and AT2 receptors. Both belong to the G-protein coupled receptor superfamily and are comprised of seven hydrophobic transmembrane segments forming α helices in the lipid bilayer of the cell membrane, an extracellular N-terminus and an intracellular N-terminus1.Two highly homologous isoforms of the receptor, AT1A and AT1B have been identified in rodents. The AT1A receptor accounts for 90% of the total binding, and is predominant in the kidney, vascular smooth muscle cells, heart, liver, and in some areas of the brain, while the AT1B receptor is found predominantly in the pituitary and adrenal glands, placenta, lung, and brain. These isoforms are pharmacologically indistinguishable and are both selectively antagonized by losartan.Through AT1 receptor, angiotensin II stimulates multiple signaling pathways, cross-talks with several tyrosine kinases, and trans-activates growth factor receptors. Phosphorylation of the receptor by GPCR kinases terminates receptor activation and promotes β-arrestin recruitment. β-arrestin-scaffolded signaling mediates ‘secondary signaling’ that involves multiple kinases that link to cell protective downstream signaling molecules2.In a model of diet-induced obese mice, AT1 receptor inhibition demonstrates beneficial pleiotropic effects. Mice receiving the treatment exhibit enhanced pancreatic duodenal homeobox 1 (PDX1) and GLP-1 pancreatic islet expression. PDX1 islets are essential for the expression of glucokinase and GLUT2. Greater GLP-1 levels, islet vascularization and reduced apoptosis and macrophage infiltration are also observed3.

合成肽

细胞外

细胞外, N末端

NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor

Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.

1 X 50 mcl

1毫克/毫升

1毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P30556

AAR-011

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

735美元