产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗血管紧张素Ⅱ受体类型1(细胞外)抗体
目录 :
AAR-011
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 14
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 5a
Gallego Delgado J, Basu Roy U, Ty M, Alique M, Fernandez Arias C, Movila A, et al. Angiotensin receptors and ?-catenin regulate brain endothelial integrity in malaria. J Clin Invest. 2016;126:4016-4029 pubmed 出版商
Aparicio Prieto M, Rodr xed guez Gallego M, Valdivia Palac xed n A, Franco Iriarte Y, Herv xe1 s Barbara G, Echevarr xed a Orella E, et al. Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl. 2022;24:139-146 pubmed 出版商
Lee C, Park H, Lee B, Jeong S, Hyun S, Choi J, et al. Novel Therapeutic Effects of Pterosin B on Ang II-Induced Cardiomyocyte Hypertrophy. Molecules. 2020;25: pubmed 出版商
Raupp D, Fernandes R, Antunes K, Perin F, Rigatto K. Impact of angiotensin II type 1 and G-protein-coupled Mas receptor expression on the pulmonary performance of patients with idiopathic pulmonary fibrosis. Peptides. 2020;133:170384 pubmed 出版商
Qi R, Li T, Chen X. The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data. Ther Adv Endocrinol Metab. 2020;11:2042018820920560 pubmed 出版商
Singh D, Singh A, Singh D, Kesavan A, Arora S, Tiwary A, et al. Enhanced oral bioavailability and anti-diabetic activity of canagliflozin through a spray dried lipid based oral delivery: a novel paradigm. Daru. 2020;28:191-208 pubmed 出版商
Parekh R, Robidoux J, Sriramula S. Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons. Cell Mol Neurobiol. 2020;40:845-857 pubmed 出版商
Jeong J, Lee J, Lim J, Cho S, An S, Lee M, et al. Soluble RAGE attenuates AngII-induced endothelial hyperpermeability by disrupting HMGB1-mediated crosstalk between AT1R and RAGE. Exp Mol Med. 2019;51:1-15 pubmed 出版商
Cho S, Cho H, Woo K, Jeong J, Lim J, Park S, et al. Nelumbo nucifera Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy. Molecules. 2019;24: pubmed 出版商
Wang M, Han W, Zhang M, Fang W, Zhai X, Guan S, et al. Long-term renal sympathetic denervation ameliorates renal fibrosis and delays the onset of hypertension in spontaneously hypertensive rats. Am J Transl Res. 2018;10:4042-4053 pubmed
Lei J, Zhang S, Wang P, Liao Y, Bian J, Yin X, et al. Long-term presence of angiotensin II type 1 receptor autoantibody reduces aldosterone production by triggering Ca2+ overload in H295R cells. Immunol Res. 2018;66:44-51 pubmed 出版商
da Silva J, Gabriel Costa D, Wang H, Ahmad S, Sun X, Varagic J, et al. Blunting of cardioprotective actions of estrogen in female rodent heart linked to altered expression of cardiac tissue chymase and ACE2. J Renin Angiotensin Aldosterone Syst. 2017;18:1470320317722270 pubmed 出版商
Tadevosyan A, Xiao J, Surinkaew S, Naud P, Merlen C, Harada M, et al. Intracellular Angiotensin-II Interacts With Nuclear Angiotensin Receptors in Cardiac Fibroblasts and Regulates RNA Synthesis, Cell Proliferation, and Collagen Secretion. J Am Heart Assoc. 2017;6: pubmed 出版商
Ismail B, dekemp R, Hadizad T, Mackasey K, Beanlands R, DaSilva J. Decreased renal AT1 receptor binding in rats after subtotal nephrectomy: PET study with [(18)F]FPyKYNE-losartan. EJNMMI Res. 2016;6:55 pubmed 出版商
图像
图像 1 :
Alomone Labs AAR-011 图像 1
Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
图像 2 :
Alomone Labs AAR-011 图像 2
Expression of Angiotensin II Receptor Type-1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panels reveals partial co-localization.
产品信息
SKU号 :
AAR-011_1_25_mcl
ParentSKU :
AAR-011
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗血管紧张素Ⅱ受体类型1(细胞外)抗体
产品描述 :
Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.
Short Description :
A兔多克隆抗体血管紧张素Ⅱ受体类型1
Negative Control :
BLP-AR011
同义词 :
血管紧张素Ⅱ受体类型1, AT1受体, AT1R, AGTR1
靶标名 :
血管紧张素Ⅱ受体类型1
Primary Category (Old) :
G-Protein Coupled Receptors > Angiotensin II Receptors > Antibodies
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
AGTR1
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse, rat, rabbit, dog - identical.
靶标/背景信息 :
Angiotensin II receptor type 1 (AT1 receptor, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (IP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by IP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor
序列文本 :
Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.7毫克/毫升
浓度 :
07毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P30556
Top Related :
AAR-012, AAR-013, AAR-011-F, ABR-011, AAR-017
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IFC Legend :
Cell surface detection of AGTR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Cell surface detection of AGTR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (2.5μg) + goat-anti-rabbit-FITC.
LCI, ICC Legend :
Expression of AGTR1 in rat C6 glioma cells - Cell surface detection of AGTR1 in live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Live intact C6 cells.
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。