产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗TRPC3抗体
目录 :
ACC-016
规格 :
1 X 50 mcl
价格 :
530美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 39
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:800; 图 5a
Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed 出版商
  • 免疫沉淀; 犬; 1:500; 图 7c
Hammers D, Sleeper M, Forbes S, Shima A, Walter G, Sweeney H. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. 2016;5: pubmed 出版商
Farfariello V, Gordienko D, Mesilmany L, Touil Y, Germain E, Fliniaux I, et al. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence. Nat Commun. 2022;13:956 pubmed 出版商
Jeong S, Oh M, Choi J, Woo J, Lee E. Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells. 2021;10: pubmed 出版商
Casas J, Meana C, Lopez Lopez J, Balsinde J, Balboa M. Lipin-1-derived diacylglycerol activates intracellular TRPC3 which is critical for inflammatory signaling. Cell Mol Life Sci. 2021;78:8243-8260 pubmed 出版商
Um K, Hahn S, Kim S, Lee Y, Birnbaumer L, Kim H, et al. TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neurons. elife. 2021;10: pubmed 出版商
Matthews A, Soni H, Robinson Freeman K, John T, Buddington R, Adebiyi A. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int J Mol Sci. 2021;22: pubmed 出版商
Azuma Y, Suzuki S, Nishiyama K, Yamaguchi T. Gastrointestinal motility modulation by stress is associated with reduced smooth muscle contraction through specific transient receptor potential channel. J Vet Med Sci. 2021;83:622-629 pubmed 出版商
Bocchero U, Falleroni F, Mortal S, Li Y, Cojoc D, LAMB T, et al. Mechanosensitivity is an essential component of phototransduction in vertebrate rods. PLoS Biol. 2020;18:e3000750 pubmed 出版商
Liu Y, Liu X, Zhang X, Deng J, Zhang J, Xing H. lncRNA DLX6-AS1 Promotes Proliferation of Laryngeal Cancer Cells by Targeting the miR-26a/TRPC3 Pathway. Cancer Manag Res. 2020;12:2685-2695 pubmed 出版商
Li S, Jiang K, Li J, Hao X, Chu W, Luo C, et al. Estrogen enhances the proliferation and migration of ovarian cancer cells by activating transient receptor potential channel C3. J Ovarian Res. 2020;13:20 pubmed 出版商
Cozart M, Phelan K, Wu H, Mu S, Birnbaumer L, Rusch N, et al. Vascular smooth muscle TRPC3 channels facilitate the inverse hemodynamic response during status epilepticus. Sci Rep. 2020;10:812 pubmed 出版商
Urban N, Schaefer M. Direct Activation of TRPC3 Channels by the Antimalarial Agent Artemisinin. Cells. 2020;9: pubmed 出版商
Choi J, Huang M, Hyun C, Oh M, Lee K, Cho C, et al. A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle. Sci Rep. 2019;9:19140 pubmed 出版商
Bernardini M, Brossa A, Chinigo G, Grolez G, Trimaglio G, Allart L, et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers (Basel). 2019;11: pubmed 出版商
Sudi S, Tanaka T, Oda S, Nishiyama K, Nishimura A, Sunggip C, et al. TRPC3-Nox2 axis mediates nutritional deficiency-induced cardiomyocyte atrophy. Sci Rep. 2019;9:9785 pubmed 出版商
Nishiyama K, Numaga Tomita T, Fujimoto Y, Tanaka T, Toyama C, Nishimura A, et al. Ibudilast attenuates doxorubicin-induced cytotoxicity by suppressing formation of TRPC3 channel and NADPH oxidase 2 protein complexes. Br J Pharmacol. 2019;176:3723-3738 pubmed 出版商
Wang Y, Qi Y, Qi Z, Tsang S. TRPC3 Regulates the Proliferation and Apoptosis Resistance of Triple Negative Breast Cancer Cells through the TRPC3/RASA4/MAPK Pathway. Cancers (Basel). 2019;11: pubmed 出版商
Picardo M, Sugimura Y, Dorst K, Kallurkar P, Akins V, Ma X, et al. Trpm4 ion channels in pre-Bötzinger complex interneurons are essential for breathing motor pattern but not rhythm. PLoS Biol. 2019;17:e2006094 pubmed 出版商
Kim E, Shotorbani P, Dryer S. TRPC6 inactivation does not affect loss of renal function in nephrotoxic serum glomerulonephritis in rats, but reduces severity of glomerular lesions. Biochem Biophys Rep. 2019;17:139-150 pubmed 出版商
Naito R, Kassai H, Sakai Y, Schönherr S, Fukaya M, Schwarzer C, et al. New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines. Front Mol Neurosci. 2018;11:439 pubmed 出版商
Hou X, Xiao H, Zhang Y, Zeng X, Huang M, Chen X, et al. Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. Cell Death Dis. 2018;9:1015 pubmed 出版商
Kim E, Yazdizadeh Shotorbani P, Dryer S. Trpc6 inactivation confers protection in a model of severe nephrosis in rats. J Mol Med (Berl). 2018;96:631-644 pubmed 出版商
Koizumi H, John T, Chia J, Tariq M, Phillips R, Mosher B, et al. Transient Receptor Potential Channels TRPM4 and TRPC3 Critically Contribute to Respiratory Motor Pattern Formation but not Rhythmogenesis in Rodent Brainstem Circuits. Eneuro. 2018;5: pubmed 出版商
Liu B, He X, Li S, Xu B, Birnbaumer L, Liao Y. Deletion of diacylglycerol-responsive TRPC genes attenuates diabetic nephropathy by inhibiting activation of the TGF?1 signaling pathway. Am J Transl Res. 2017;9:5619-5630 pubmed
Shimauchi T, Numaga Tomita T, Ito T, Nishimura A, Matsukane R, Oda S, et al. TRPC3-Nox2 complex mediates doxorubicin-induced myocardial atrophy. JCI Insight. 2017;2: pubmed 出版商
Wang X, Sun W, Fu J, Huang J, Yu C, Underwood M, et al. Impairment of Coronary Endothelial Function by Hypoxia-Reoxygenation Involves TRPC3 Inhibition-mediated KCa Channel Dysfunction: Implication in Ischemia-Reperfusion Injury. Sci Rep. 2017;7:5895 pubmed 出版商
Wang B, Xiong S, Lin S, Xia W, Li Q, Zhao Z, et al. Enhanced Mitochondrial Transient Receptor Potential Channel, Canonical Type 3-Mediated Calcium Handling in the Vasculature From Hypertensive Rats. J Am Heart Assoc. 2017;6: pubmed 出版商
Jia S, Rodriguez M, Williams A, Yuan J. Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation. Sci Rep. 2017;7:5075 pubmed 出版商
Liu N, Zhuang Y, Zhou Z, Zhao J, Chen Q, Zheng J. NF-?B dependent up-regulation of TRPC6 by A? in BV-2 microglia cells increases COX-2 expression and contributes to hippocampus neuron damage. Neurosci Lett. 2017;651:1-8 pubmed 出版商
Sun Y, Zhang H, Selvaraj S, Sukumaran P, Lei S, Birnbaumer L, et al. Inhibition of L-Type Ca2+ Channels by TRPC1-STIM1 Complex Is Essential for the Protection of Dopaminergic Neurons. J Neurosci. 2017;37:3364-3377 pubmed 出版商
Mamenko M, Boukelmoune N, Tomilin V, Zaika O, Jensen V, O Neil R, et al. The renal TRPV4 channel is essential for adaptation to increased dietary potassium. Kidney Int. 2017;91:1398-1409 pubmed 出版商
Chen M, Liu J, Lu Y, Duan C, Lu L, Gao G, et al. Age-dependent alpha-synuclein accumulation is correlated with elevation of mitochondrial TRPC3 in the brains of monkeys and mice. J Neural Transm (Vienna). 2017;124:441-453 pubmed 出版商
Storck H, Hild B, Schimmelpfennig S, Sargin S, Nielsen N, Zaccagnino A, et al. Ion channels in control of pancreatic stellate cell migration. Oncotarget. 2017;8:769-784 pubmed 出版商
Alvarez Miguel I, Cidad P, Pérez García M, Lopez Lopez J. Differences in TRPC3 and TRPC6 channels assembly in mesenteric vascular smooth muscle cells in essential hypertension. J Physiol. 2017;595:1497-1513 pubmed 出版商
Kim J, Xie J, Hwang K, Wu Y, Oliver N, Eom M, et al. Klotho May Ameliorate Proteinuria by Targeting TRPC6 Channels in Podocytes. J Am Soc Nephrol. 2017;28:140-151 pubmed 出版商
Wang Q, Leo M, Narayanan D, Kuruvilla K, Jaggar J. Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries. Am J Physiol Cell Physiol. 2016;310:C1001-9 pubmed 出版商
Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed 出版商
Ampem P, Smedlund K, Vazquez G. Pharmacological evidence for a role of the transient receptor potential canonical 3 (TRPC3) channel in endoplasmic reticulum stress-induced apoptosis of human coronary artery endothelial cells. Vascul Pharmacol. 2016;76:42-52 pubmed 出版商
图像
图像 1 :
Alomone Labs ACC-016 图像 1
Western blot analysis of rat heart membranes: - 1. Anti-TRPC3 Antibody (#ACC-016), (1:200).2. Anti-TRPC3 Antibody, preincubated with TRPC3 Blocking Peptide (#BLP-CC016).
图像 2 :
Alomone Labs ACC-016 图像 2
Expression of TRPC3 in rat pituitary gland - Immunohistochemical staining of rat pituitary gland paraffin embedded sections using Anti-TRPC3 Antibody (#ACC-016), (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
图像 3 :
Alomone Labs ACC-016 图像 3
Western blotanalysisof rat heart membranes: - 1.Anti-TRPC3Antibody (#ACC-016) (1:200).2. Anti-TRPC3 Antibody preincubated with the control peptide antigen.
产品信息
SKU号 :
ACC-016_1_50_mcl
ParentSKU :
ACC-016
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗TRPC3抗体
产品描述 :
Anti-TRPC3 Antibody (#ACC-016) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples.
Short Description :
A Rabbit Polyclonal Antibody to TRPC3 Channel
Negative Control :
BLP-CC016
同义词 :
TRPC3, Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
靶标名 :
TRPC3 Channel
Primary Category (Old) :
Ion Channels > TRP Channels > TRPC Channels > Antibodies to TRPC Channels
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, 免疫沉淀, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
TRPC3
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Human, rat - 13/14 amino acid residues identical.
靶标/背景信息 :
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:1. TRPC12. TRPC4 and TRPC53. TRPC3, TRPC6, TRPC74. TRPC2.2,5They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7
免疫原 :
合成肽
区域 :
intracellular
Loop :
Intracellular, C-terminus
序列 :
HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3
序列文本 :
Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Primary Size (Size+Units) :
1 X 50 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
Q9QZC1
Top Related :
ACC-017, ACC-010, ACC-018, ACC-020, ACC-120
储存和重组 :
Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
50
单位 :
mcl
标价美国[美元] :
530美元
ICC Legend :
Expression of TRPC3 in rat C6 brain glioma cells - Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized rat C6 brain glioma cells. A. Staining using Anti-TRPC3 Antibody (#ACC-016), (1:500) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。