抗体

抗TRPC4抗体

ACC-018

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

ACC-018

Primary Antibody>Intracellular>Non-Labeled

Insert

抗TRPC4抗体

Anti-TRPC4 Antibody (#ACC-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC4 from human, mouse, and rat samples.

A Rabbit Polyclonal Antibody to TRPC4 Channel

BLP-CC018

TRPC4, Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1

TRPC4 Channel

Ion Channels > TRP Channels > TRPC Channels > Antibodies to TRPC Channels

未共轭

免疫细胞化学, IF, 免疫组化, 免疫沉淀, 免疫印迹

兔

多克隆

兔IgG

TRPC4

人类(试验);小鼠(试验);大鼠(试验)

Rat, human - 15/16 amino acid residues identical; bovine - 11/15 amino acid residues identical.

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4  The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5  They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by calcium store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells. 6

合成肽

intracellular

Intracellular, C-terminus

(C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4

Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5). Intracellular, C-terminus.

1 X 25 mcl

0.4毫克/毫升

04毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q9QUQ5

ACC-010, ACC-016, ACC-017, ACC-020, ACC-119

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Immunoprecipitation of rat brain lysate: - 1. Rat brain lysate.2. Lysate immunoprecipitated with Anti-TRPC4 Antibody (#ACC-018), (6 µg).3. Lysate immunoprecipitated with pre-immune rabbit serum.The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain.Western blot analysis was performed with Anti-TRPC4 Antibody.